Mouse Anti-JMJD6 Recombinant Antibody (VS7-0425-WR170) (CAT#: VS7-0425-WR170)
This product is a mouse IgG antibody that binds to human JMJD6. The antibody can be used for WB, IF, and ICC assays. It cross reacts with mouse, rat, bovine, and dog.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line PC-3
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59156/1020_D11_1_selected.jpg
(Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59156/1020_D11_1_selected.jpg
Figure 2 IHC staining of human lymph node
(Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/ihc_selected.jpg
(Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/ihc_selected.jpg
Figure 3 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59156/1043_F5_1_red_green.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59156/1043_F5_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59156/993_D11_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59156/993_D11_1_red_green.jpg
Figure 5 Cerebral cortex
(Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_B_7_5.jpg
(Glial cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_B_7_5.jpg
Figure 6 Colon
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_8_3.jpg
(Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_8_3.jpg
Figure 7 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_7_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_7_5.jpg
Figure 8 Testis
(Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_4_6.jpg
(Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Round or early spermatids
Staining:Medium
Intensity: Strong
Quantity: <25%
Sertoli cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_4_6.jpg
Figure 9 Lymph node
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_8_8.jpg
(Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4548/11495_A_8_8.jpg
Figure 10 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000070495-JMJD6
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000070495-JMJD6
Specifications
- Immunogen
- Recombinant human JMJD6 (N-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human JMJD6
- Species Reactivity
- Human, Mouse, Rat, Bovine, Dog
- Molecule Class
- Nuclear Protein
- Applications
- WB, IF, ICC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:1000 - 1:2000
Immunofluorescence: 1:100 - 1:500
Target
- Alternative Names
- JMJD6; jumonji domain containing 6; PSR; PTDSR; PTDSR1; bifunctional arginine demethylase and lysyl-hydroxylase JMJD6; histone arginine demethylase JMJD6; jmjC domain-containing protein 6; jumonji domain-containing protein 6; lysyl-hydroxylase JMJD6; pept
- Gene ID
- 23210
- UniProt ID
- Q6NYC1
- Long Name
- Jumonji Domain Containing 6, Arginine Demethylase And Lysine Hydroxylase
- Cellular Localization
- Cytoplasm; Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001074930.1
- Function
- Chromatin regulator; Developmental protein; Dioxygenase; Oxidoreductase; RNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-360 | Recombinant Human Anti-Human JMJD6 Antibody, clone D277 | IP, IF, ELISA, IP-MS | IgG |
| PABL-623 | Mouse Anti-JMJD6 Recombinant Antibody | WB, IHC, IF, FuncS | Mouse IgG |
| FAMAB-1374CQ | Human Anti-JMJD6 Recombinant Antibody (clone APSR-14.4) | IP, WB, IF | Human IgG1 |
| ZG-0522J | Mouse Anti-JMJD6 Recombinant Antibody (ZG-0522J) | WB, ICC | Mouse IgG |
| VS4-WK261 | Mouse Anti-JMJD6 Recombinant Antibody (VS4-WK261) | ELISA, WB, ICC | Mouse IgG |
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