Mouse Anti-KDM4A Monoclonal Antibody (VS7-0425-WR201)
CAT#: VS7-0425-WR201
This product is a mouse IgG antibody that binds to human KDM4A. The antibody can be used for WB, IHC-P, IF, ICC, and ELISA assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/75_D3_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/74_D3_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_B_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000066135-KDM4A
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Specifications
- Immunogen
- Recombinant human KDM4A fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KDM4A
- Species Reactivity
- Human
- Molecule Class
- Demethylase
- Applications
- WB, IHC-P, IF, ICC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KDM4A; lysine (K)-specific demethylase 4A; JMJD2; JHDM3A; JMJD2A; TDRD14A; lysine-specific demethylase 4A; jmjC domain-containing histone demethylation protein 3A; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji do
- Gene ID
- 9682
- UniProt ID
- O75164
- Long Name
- Lysine Demethylase 4A
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_055478.2
- Function
- Chromatin regulator; Dioxygenase; Oxidoreductase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "KDM4A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-366 | Recombinant Human Anti-Human KDM4A Antibody Fab Fragment, clone D266 | IP, IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1431MZ | Recombinant Mouse Anti-Human KDM4A Antibody (clone 6I2) | ELISA, ICC, IF, IHC-P, WB | Mouse antibody |
| ZG-0521J | Mouse Anti-KDM4A Recombinant Antibody (ZG-0521J) | WB, IHC, IF, ELISA | Mouse IgG |
| VS3-WK56 | Mouse Anti-KDM4A Recombinant Antibody (clone E7-H1) | WB, ELISA | Mouse IgG1 |
| VS3-XY963 | Mouse Anti-KDM4A Recombinant Antibody (clone 5H1) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| VS3-FY791 | Recombinant Rabbit Anti-KDM4A Antibody (clone R01-7F2) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3752 | Anti-KDM4A Immunohistochemistry Kit | IHC |
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