Mouse Anti-KDM4A Recombinant Antibody (ZG-0521J)
CAT#: ZG-0521J
This product is a mouse antibody that recognizes KDM4A.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-KDM4A Recombinant Antibody (ZG-0521J) in WB
Western Blot analysis using JMJD2A Monoclonal Antibody against HEK293 (1) and JMJD2A-hIgGFc transfected HEK293 (2) cell lysate.
IHC
Figure 2 Mouse Anti-KDM4A Recombinant Antibody (ZG-0521J) in IHC
Immunohistochemistry analysis of paraffin-embedded colon cancer tissues (left) and human larynx cancer tissues (right) with DAB staining using JMJD2A Monoclonal Antibody.
IF
Figure 3 Mouse Anti-KDM4A Recombinant Antibody (ZG-0521J) in IF
Immunofluorescence analysis of NTERA-2 cells using JMJD2A Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/75_D3_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/74_D3_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_B_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7610/21324_A_4_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000066135-KDM4A
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Specifications
- Immunogen
- Purified recombinant fragment of human JMJD2A expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KDM4A
- Species Reactivity
- Human
- Applications
- WB, IHC, IF, ELISA
Product Property
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Western Blot, Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- JMJD2; JHDM3A; JMJD2A; TDRD14A; lysine-specific demethylase 4A; [histone H3]-trimethyl-L-lysine(36) demethylase 4A; [histone H3]-trimethyl-L-lysine(9) demethylase 4A; jmjC domain-containing histone demethylation protein 3A; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji domain containing 2A; jumonji domain-containing protein 2A; lysine (K)-specific demethylase 4A; tudor domain containing 14A
- Gene ID
- 9682
- UniProt ID
- O75164
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "KDM4A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-366 | Recombinant Human Anti-Human KDM4A Antibody Fab Fragment, clone D266 | IP, IF, ELISA, IP-MS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK56 | Mouse Anti-KDM4A Recombinant Antibody (clone E7-H1) | WB, ELISA | Mouse IgG1 |
| VS3-WK137 | Mouse Anti-KDM4A Recombinant Antibody (clone G4-E10) | WB, ELISA | Mouse IgG1 |
| VS3-FY791 | Recombinant Rabbit Anti-KDM4A Antibody (clone R01-7F2) | WB, IP | Rabbit IgG |
| VS7-0425-WR200 | Mouse Anti-KDM4A Recombinant Antibody (clone 6E10G4) | ICC, FC | Mouse IgG1 |
| VS7-0425-WR201 | Mouse Anti-KDM4A Monoclonal Antibody (VS7-0425-WR201) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3752 | Anti-KDM4A Immunohistochemistry Kit | IHC |
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