Rabbit Anti-KLK3 Recombinant Antibody (clone 7D4)

Name: Rabbit Anti-KLK3 Recombinant Antibody (clone 7D4)
SKU: ZG-0787J
Rating: 5 (1 reviews)

CAT#: ZG-0787J

This product is a rabbit antibody that recognizes KLK3.

Tested Data Gene Expression

IHC

Figure 1 Rabbit Anti-KLK3 Antibody (ZG-0787J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

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Normal Tissue Figure 1 IHC staining of human prostate

Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/764/2093_A_1_5_selected.jpg

RNA Expression Figure 2 RNA cell line category: Group enriched (BEWO, NB-4, RPMI-8226, SH-SY5Y, U-2197)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000142515-KLK3

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Specifications

Immunogen
A synthesized peptide derived from human PSA/KLK3

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human KLK3

Species Reactivity
Human

Clone
7D4

Applications
ELISA, IHC

Conjugate
Uconjugated

Related Disease
Cancer

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200

Target

Alternative Names
APS; PSA; hK3; KLK2A1; prostate-specific antigen; P-30 antigen; gamma-seminoprotein; kallikrein-3; semenogelase; seminin

Gene ID
354

UniProt ID
P07288

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - ZG-0787J.pdf

MSDS

MSDS - ZG-0787J.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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