AbPlus™ Anti-KRT5 Magnetic Beads (VS-0724-YC1518)
CAT#: VS-0724-YC1518
The AbPlus Anti-KRT5 Magnetic Beads (VS-0724-YC1518) is an innovative affinity resin which is bound with anti-KRT5 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of KRT5 protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line hTCEpi
Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/1790_D3_5_selected.jpg
Normal Tissue
Figure 2 High expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_2_6_val_selected.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: High Intensity: Strong Quantity:>75% Hair follicles Staining: High Intensity: Strong Quantity: 75%-25% Sebaceous glands Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HaCaT, HBEC3-KT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186081-KRT5
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-KRT5 specific antibody
- Target
- KRT5
- Immunogen
- Synthetic peptide corresponding to the center region of the human Cytokeratin 5/KRT5.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
- Alternative Names
- Keratin 5; Keratin 5 (Epidermolysis Bullosa Simplex, Dowling-Meara/Kobner/Weber-Cockayne Types); Epidermolysis Bullosa Simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; Type-II Keratin Kb5; Keratin 5, Type II; Cytokeratin-5; CK-5; K5; Keratin, Type II Cytoskeletal 5;
- Gene ID
- 3852
- UniProt ID
- P13647
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "KRT5"
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|---|---|---|---|
| MOB-1997z | Mouse Anti-KRT5 Recombinant Antibody (clone 9H9) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| MOB-1510MZ | Recombinant Mouse Anti-Human KRT5 Antibody (clone YN37) | ICC, IHC-Fr, IHC-P | Mouse antibody |
| CAT | Product Name | Application | Type |
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| MOR-1990 | Hi-Affi™ Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (DS1990AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
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| MRO-0448-CN | Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (SY88-19) | WB, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| MRO-0449-CN | Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (CBACN-186) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0459F | Mouse Anti-KRT5 Recombinant Antibody (ZG-0459F) | IHC, IF, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
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| VS13-YC636 | CytoStream™ Rabbit Anti-KRT5 Recombinant Antibody (VS13-YC636) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3874 | Anti-KRT5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3875 | Anti-Mouse KRT5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3876 | Anti-Rat KRT5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3877 | Anti-Bovine KRT5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3878 | Anti-Porcine KRT5 Immunohistochemistry Kit | IHC |
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