Mouse Anti-KRT5 Recombinant Antibody (ZG-0459F)
CAT#: ZG-0459F
This product is a mouse antibody that recognizes KRT5.
Tested Data
Gene Expression
IHC
Figure 1 Mouse Anti-KRT5 Recombinant Antibody (ZG-0459F) in IHC.
Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma (A), normal hepatocytes (B), colon adenocarcinoma, and normal gastric tissue (D), using cytokeratin (Pan) Monoc for DAB staining to show the cytoplasm And film positioning
IF
Figure 2 Mouse Anti-KRT5 Recombinant Antibody (ZG-0459F) in IF.
Confocal immunofluorescence analysis of methanol-fixed Eca-109 cells using cytokeratin (Pan) monoclonal antibody (green) showed cytoplasmic localization. Blue: DRAQ5 fluorescent DNA dye.
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Subcellular Location
Figure 1 IF staining of human cell line hTCEpi
Immunofluorescent staining of human cell line hTCEpi shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/1790_D3_5_selected.jpg
Normal Tissue
Figure 2 High expression in skin 1
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1_selected.jpg
Normal Tissue
Figure 3 Low expression in skeletal muscle
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_2_6_val_selected.jpg
Normal Tissue
Figure 4 Skin
Cells in basal layer Staining: High Intensity: Strong Quantity:>75% Cells in spinous layer Staining: Medium Intensity: Moderate Quantity: 75%-25% Eccrine glands Staining: High Intensity: Strong Quantity:>75% Hair follicles Staining: High Intensity: Strong Quantity: 75%-25% Sebaceous glands Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/59479/133810_B_7_1.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (HaCaT, HBEC3-KT, hTCEpi)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000186081-KRT5
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Specifications
- Immunogen
- Recombinant fragment of Cytokeratin 5 expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human KRT5
- Species Reactivity
- Human
- Applications
- Immunohistochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/200-1/1000
Immunofluorescence: 1/200-1/1000
ELISA: 1/10000
Target
- Alternative Names
- Keratin 5; Keratin 5 (Epidermolysis Bullosa Simplex, Dowling-Meara/Kobner/Weber-Cockayne Types); Epidermolysis Bullosa Simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; Type-II Keratin Kb5; Keratin 5, Type II; Cytokeratin-5; CK-5; K5; Keratin, Type II Cytoskeletal 5; KRT5; Keratin; type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II keratin Kb5
- Gene ID
- 3852
- UniProt ID
- P13647
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "KRT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1990 | Hi-Affi™ Recombinant Rabbit Anti-KRT5 Monoclonal Antibody (DS1990AB) | IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-XY997 | Mouse Anti-KRT5 Recombinant Antibody (clone 7H8C4) | ELISA, WB, IHC, ICC | Mouse IgG1 |
| VS3-XY998 | Mouse Anti-KRT5 Recombinant Antibody (clone 10C11E6) | ICC, IHC | Mouse IgG1 |
| VS3-XY999 | Mouse Anti-KRT5 Recombinant Antibody (clone 3C8) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-XY1000 | Mouse Anti-KRT5 Recombinant Antibody (clone 3E2F1) | ELISA, WB | Mouse IgG1 |
| VS11-QX95 | Mouse Anti-KRT5 Recombinant Antibody (clone AbM58019-7H4) | ELISA, IHC | Mouse IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1518 | AbPlus™ Anti-KRT5 Magnetic Beads (VS-0724-YC1518) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC636 | CytoStream™ Rabbit Anti-KRT5 Recombinant Antibody (VS13-YC636) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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