CytoStream™ Mouse Anti-LAMP2 Recombinant Antibody (VS13-YC645)
CAT#: VS13-YC645
The CytoStream™ Mouse Anti-LAMP2 Recombinant Antibody is specific for human LAMP2. LAMP2, coding for lysosomal-associated glycoproteins, provides selectin carbohydrate ligands, potentially relatable to metastasis and lysosome protection and adhesion. Multiple spliced variants encode distinct proteins associated with diseases like Danon disease. The anti-LAMP2 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/598_H2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5272/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005893-LAMP2
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Specifications
- Immunogen
- Recombinant human LAMP2, aa 1-300.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human LAMP2
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
- Related Disease
- Danon Disease and Atrial Standstill
Product Property
- Clonality
- Monoclonal
- Purification
- Protein G purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 50% Glycerol and 0.2% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/100
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Lysosomal Associated Membrane Protein 2 CD107 Antigen-Like Family Member B; LAMP-2; LGP-96; Lysosome-Associated Membrane Glycoprotein
- Gene ID
- 3920
- UniProt ID
- P13473
- Long Name
- Lysosomal Associated Membrane Protein 2
- Sequence Similarities
- Belongs to the LAMP family.
- Cellular Localization
- Cell membrane; Lysosome membrane; Endosome membrane; Autophagosome membrane
- Protein Refseq
- NP_001116078.1; NP_002285.1; NP_054701.1
- Function
- Plays an important role in chaperone-mediated autophagy, a process that mediates lysosomal degradation of proteins in response to various stresses and as part of the normal turnover of proteins with a long biological half-live (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125, PubMed:27628032).
Functions by binding target proteins, such as GAPDH and MLLT11, and targeting them for lysosomal degradation (PubMed:8662539, PubMed:11082038, PubMed:18644871, PubMed:24880125).
Plays a role in lysosomal protein degradation in response to starvation (By similarity).
Required for the fusion of autophagosomes with lysosomes during autophagy (PubMed:27628032).
Cells that lack LAMP2 express normal levels of VAMP8, but fail to accumulate STX17 on autophagosomes, which is the most likely explanation for the lack of fusion between autophagosomes and lysosomes (PubMed:27628032).
Required for normal degradation of the contents of autophagosomes (PubMed:27628032).
Required for efficient MHCII-mediated presentation of exogenous antigens via its function in lysosomal protein degradation; antigenic peptides generated by proteases in the endosomal/lysosomal compartment are captured by nascent MHCII subunits (PubMed:20518820).
Is not required for efficient MHCII-mediated presentation of endogenous antigens (PubMed:20518820).
Isoform LAMP-2C
Modulates chaperone-mediated autophagy. Decreases presentation of endogenous antigens by MHCII. Does not play a role in the presentation of exogenous and membrane-derived antigens by MHCII.
(Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "LAMP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2011 | Hi-Affi™ Recombinant Rabbit Anti-LAMP2 Monoclonal Antibody (DS2011AB) | WB, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2007-CN | Rabbit Anti-LAMP2 Polyclonal Antibody (MRO-2007-CN) | WB, IHC | Rabbit IgG |
| VS3-XY1002 | Mouse Anti-LAMP2 Recombinant Antibody (clone C9-9) | WB, ICC | Mouse IgG2b |
| VS3-FY838 | Recombinant Rabbit Anti-LAMP2 Antibody (clone R02-3F4) | WB, IHC-P | Rabbit IgG |
| VS3-FY839 | Recombinant Rabbit Anti-LAMP2 Antibody (clone R03-0B5) | WB, IHC-P | Rabbit IgG |
| VS3-FY840 | Recombinant Rabbit Anti-LAMP2 Antibody (clone R03-2J8) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1043 | AbPlus™ Anti-LAMP2 Magnetic Beads (VS-0724-YC1043) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY302 | Mouse Anti-NHP LAMP2 Recombinant Antibody (clone H4B4) | WB, IF, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1225 | Anti-LAMP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3941 | Anti-Mouse LAMP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3940 | Anti-Human LAMP2 Immunohistochemistry Kit | IHC |
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