Mouse Anti-NHP LAMP2 Recombinant Antibody (clone H4B4)
CAT#: VS-1024-XY302
Designed for the detection of LAMP2, this mouse monoclonal antibody demonstrates reactivity with human, mouse, and non-human primate tissues. Its robust performance in various applications, including Western blot (WB), immunofluorescence (IF), and flow cytometry (FC), makes it an invaluable tool for elucidating LAMP2 expression and function. With a focus on specificity and reliability, this antibody is essential for researchers investigating lysosomal dynamics in diverse biological contexts.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/598_H2_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human liver
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5272/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Collecting ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Strong
Quantity: <25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_6_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29100/89892_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000005893-LAMP2
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Specifications
- Immunogen
- Adherent spleen cells
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- LAMP2
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- H4B4
- Applications
- WB, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IF: 2-10 µg/mL, FC: 5-10 µg/mL
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "LAMP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2011 | Hi-Affi™ Recombinant Rabbit Anti-LAMP2 Monoclonal Antibody (DS2011AB) | WB, ICC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2007-CN | Rabbit Anti-LAMP2 Polyclonal Antibody (MRO-2007-CN) | WB, IHC | Rabbit IgG |
| VS3-FY838 | Recombinant Rabbit Anti-LAMP2 Antibody (clone R02-3F4) | WB, IHC-P | Rabbit IgG |
| VS3-FY839 | Recombinant Rabbit Anti-LAMP2 Antibody (clone R03-0B5) | WB, IHC-P | Rabbit IgG |
| VS3-FY840 | Recombinant Rabbit Anti-LAMP2 Antibody (clone R03-2J8) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC646 | Rabbit Anti-LAMP2 Recombinant Antibody (VS13-YC646) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1043 | AbPlus™ Anti-LAMP2 Magnetic Beads (VS-0724-YC1043) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1225 | Anti-LAMP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3941 | Anti-Mouse LAMP2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3940 | Anti-Human LAMP2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC645 | CytoStream™ Mouse Anti-LAMP2 Recombinant Antibody (VS13-YC645) | WB, ICC, IF, IHC-P, FC | Mouse IgG1 |
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