Rabbit Anti-LAT Recombinant Antibody (clone 2E4)
CAT#: ZG-0789J
This product is a rabbit antibody that recognizes LAT.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-LAT Antibody (ZG-0789J) in WB
Western Blot
Positive WB detected in:Jurkat whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate(treated with EGF or Pervanadate)
All lanes: Phospho-LAT antibody at 2.9 μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 38 KDa
Observed band size: 38 KDa
IHC
Figure 2 Rabbit Anti-LAT Antibody (ZG-0789J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC
Figure 3 Rabbit Anti-LAT Antibody (ZG-0789J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 4 Rabbit Anti-LAT Antibody (ZG-0789J) in IF
Immunofluorescence staining of Hela cells with this product at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
IP
Figure 5 Rabbit Anti-LAT Antibody (ZG-0789J) in IP
Immunoprecipitating Phospho-LAT in A549 whole cell lysate
Lane 1: Rabbit control IgG (1μg) in A549 whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0789J (3μg)+ A549 whole cell lysate (1mg)
Lane 3: A549 whole cell lysate (20μg)
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Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to plasma membrane & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/1770_C1_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human tonsil
High expression. RNA expression: 29.0 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebral cortex
Low expression. RNA expression: 11.9 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_B_8_5_val_selected.jpg
Normal Tissue
Figure 4 IHC staining of human lymph node
High expression. RNA expression: 65.9 nTPM
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2223/ihc_selected.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Leydig cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11157/26847_A_4_6.jpg
Normal Tissue
Figure 9 Spleen
Cells in red pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in white pulp
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2223/5874_B_8_4.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (HEL, HMC-1, MOLT-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000213658-LAT
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-LAT (Y191)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LAT
- Species Reactivity
- Human
- Clone
- 2E4
- Applications
- ELISA, WB, IHC, IF, IP
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200, IP:1:200-1:1000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-0316MZ | Recombinant Mouse Anti-Human LAT Antibody (clone MAT-2) | IHC | Mouse antibody |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2017 | Hi-Affi™ Recombinant Rabbit Anti-LAT Monoclonal Antibody (DS2017AB) | FC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0165-YC-S(P) | Recombinant Human Anti-LAT Antibody scFv Fragment (A1) | ELISA | Human scFv |
| HPAB-J0166-YC-S(P) | Recombinant Human Anti-LAT Antibody scFv Fragment (C10) | ELISA | Human scFv |
| HPAB-J0167-YC-S(P) | Recombinant Human Anti-LAT Antibody scFv Fragment (D3) | ELISA | Human scFv |
| HPAB-J0168-YC-S(P) | Recombinant Human Anti-LAT Antibody scFv Fragment (G10) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0165-YC-F(E) | Recombinant Human Anti-LAT Antibody Fab Fragment (A1) | ELISA | Human Fab |
| HPAB-J0166-YC-F(E) | Recombinant Human Anti-LAT Antibody Fab Fragment (C10) | ELISA | Human Fab |
| HPAB-J0167-YC-F(E) | Recombinant Human Anti-LAT Antibody Fab Fragment (D3) | ELISA | Human Fab |
| HPAB-J0168-YC-F(E) | Recombinant Human Anti-LAT Antibody Fab Fragment (G10) | ELISA | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC488 | Recombinant Anti-LAT Vesicular Antibody, EV Displayed (VS-0425-YC488) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3951 | Anti-LAT Immunohistochemistry Kit | IHC | |
| VS-0525-XY3952 | Anti-Mouse LAT Immunohistochemistry Kit | IHC |
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