Rabbit Anti-LDHB Antibody (VS-0924-YC66) - Cancer Stem Cell Marker (CAT#: VS-0924-YC66)
The product is an anti-LDHB antibody (VS-0924-YC66), designed to target the human LDHB protein, a marker for cancer stem cells (CSCs). This antibody is ideal for detecting LDHB in CSC tissues or cells using various techniques, including Immunohistochemistry (1/1000-1/2500), Immunofluorescence (0.5-2 µg/mL), and Western blot (0.1-0.5 µg/mL).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19007/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19007/if_selected.jpg

Figure 2 IHC staining of human kidney
(Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19007/43808_A_7_5_selected.jpg
(Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19007/43808_A_7_5_selected.jpg

Figure 3 IHC staining of human pancreas
(Low expression. RNA expression: 370.3 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_3_3_val_selected.jpg
(Low expression. RNA expression: 370.3 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_3_3_val_selected.jpg

Figure 4 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_B_7_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_B_7_5.jpg

Figure 5 Colon
(Endocrine cells
Staining: Medium
Intensity: Moderate
Enterocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_9_3.jpg
(Endocrine cells
Staining: Medium
Intensity: Moderate
Enterocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_9_3.jpg

Figure 6 Liver
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_9_4.jpg
(Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_9_4.jpg

Figure 7 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_4_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_4_6.jpg

Figure 8 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_7_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_7_8.jpg

Figure 9 RNA cell line category: Cell line enhanced (RPTEC TERT1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111716-LDHB
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111716-LDHB
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LDHB
- Species Reactivity
- Human
- Applications
- IHC, WB, IF
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.2 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/1000-1/2500
Western Blot (WB): 0.1-0.5 µg/mL
Immunofluorescence (IF): 0.5-2 µg/mL
Target
- Alternative Names
- LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281
- Gene ID
- 3945
- UniProt ID
- P07195
- Long Name
- Lactate dehydrogenase B
- Cellular Localization
- #Cytoplasm
#Membrane
#Mitochondrion
#Mitochondrion inner membrane
- Function
- #Oxidoreductase
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Downloads
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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