Rabbit Anti-LDHB Antibody (VS-0924-YC66) - Cancer Stem Cell Marker

Name: Rabbit Anti-LDHB Antibody (VS-0924-YC66) - Cancer Stem Cell Marker
SKU: VS-0924-YC66
Rating: 5 (1 reviews)

CAT#: VS-0924-YC66

The product is an anti-LDHB antibody (VS-0924-YC66), designed to target the human LDHB protein, a marker for cancer stem cells (CSCs). This antibody is ideal for detecting LDHB in CSC tissues or cells using various techniques, including Immunohistochemistry (1/1000-1/2500), Immunofluorescence (0.5-2 µg/mL), and Western blot (0.1-0.5 µg/mL).

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19007/if_selected.jpg

Normal Tissue Figure 2 IHC staining of human kidney

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19007/43808_A_7_5_selected.jpg

Normal Tissue Figure 3 IHC staining of human pancreas

Low expression. RNA expression: 370.3 nTPM

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_3_3_val_selected.jpg

Normal Tissue Figure 4 Cerebral cortex

Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_B_7_5.jpg

Normal Tissue Figure 5 Colon

Endocrine cells
Staining: Medium
Intensity: Moderate
Enterocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_9_3.jpg

Normal Tissue Figure 6 Liver

Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_9_4.jpg

Normal Tissue Figure 7 Testis

Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_4_6.jpg

Normal Tissue Figure 8 Lymph node

Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4641/15448_A_7_8.jpg

RNA Expression Figure 9 RNA cell line category: Cell line enhanced (RPTEC TERT1)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111716-LDHB

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Specifications

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human LDHB

Species Reactivity
Human

Applications
IHC, WB, IF

Product Property

Clonality
Polyclonal

Purification
Affinity purified using the PrEST antigen as affinity ligand

Concentration
0.2 mg/mL

Buffer
PBS, pH 7.2, with 40% glycerol

Preservative
0.02% Sodium azide

Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
Immunohistochemistry (IHC): 1/1000-1/2500
Western Blot (WB): 0.1-0.5 µg/mL
Immunofluorescence (IF): 0.5-2 µg/mL

Target

Alternative Names
LDH-B; LDH-H; LDHBD; TRG-5; HEL-S-281

Gene ID
3945

UniProt ID
P07195

Long Name
Lactate dehydrogenase B

Cellular Localization
#Cytoplasm
#Membrane
#Mitochondrion
#Mitochondrion inner membrane

Function
#Oxidoreductase

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Downloadable Resources

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Datasheet

Datasheet - VS-0924-YC66.pdf

MSDS

MSDS - VS-0924-YC66.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

CAT
Product Name

Secondary Antibody

CAT
Product Name

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