Mouse Anti-LDL Recombinant Antibody (clone 4E4) (CAT#: FAMAB-0186JF)

The recombinant monoclonal antibody is specific for acetylated LDL (AcLDL) and malondialdehyde-treated LDL (MDA-LDL). This mouse anti-LDL antibody (4E4) specifically binds to modified LDL present in human atherosclerotic lesions.

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ELISA

Figure 1 Anti-OxLDL monoclonal antibodies 4E4 distinguish between human OxLDL and native LDL.

Figure 1 Anti-OxLDL monoclonal antibodies 4E4 distinguish between human OxLDL and native LDL.

Data are expressed as mean optical density ± standard error oftriplicates.

Boyd, H. C., Gown, A. M., Wolfbauer, G., & Chait, A. (1989). Direct evidence for a protein recognized by a monoclonal antibody against oxidatively modified LDL in atherosclerotic lesions from a Watanabe heritable hyperlipidemic rabbit. The American journal of pathology, 135(5), 815.

IF

Figure 2 Immunofluorescent detection of LDL in atherosclerotic lesions.

Figure 2 Immunofluorescent detection of LDL in atherosclerotic lesions.

Original magnification X175.

Boyd, H. C., Gown, A. M., Wolfbauer, G., & Chait, A. (1989). Direct evidence for a protein recognized by a monoclonal antibody against oxidatively modified LDL in atherosclerotic lesions from a Watanabe heritable hyperlipidemic rabbit. The American journal of pathology, 135(5), 815.

WB

Figure 3 Reproduction of a Western blot analysis of the degree of LDL fragmentation and aggregation associated with different times of oxidation.

Figure 3 Reproduction of a Western blot analysis of the degree of LDL fragmentation and aggregation associated with different times of oxidation.

Reproduction of an agarose gel electrophoretogram of the samples separated by Western blot.

Lopes-Virella, M. F., Koskinen, S., Mironova, M., Horne, D., Klein, R., Chassereau, C., ... & Virella, G. (2000). The preparation of copper-oxidized LDL for the measurement of oxidized LDL antibodies by EIA. Atherosclerosis, 152(1), 107-115.

IHC

Figure 4 Immunohistology of the adrenal gland stained with anti-LDL receptor antibody.

Figure 4 Immunohistology of the adrenal gland stained with anti-LDL receptor antibody.

High power in zona fasciculata (X200).

Fong, L. G., Bonney, E., Kosek, J. C., & Cooper, A. D. (1989). Immunohistochemical localization of low density lipoprotein receptors in adrenal gland, liver, and intestine. The Journal of clinical investigation, 84(3), 847-856.


Specifications

  • Immunogen
  • Acetylated LDL
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human LDL
  • Species Reactivity
  • Human
  • Clone
  • 4E4
  • Applications
  • WB, ELISA, IHC, IF
  • Related Disease
  • Atherosclerosis

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The LDL antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence.

Target

  • Alternative Names
  • Low-density lipoprotein; LDL; Ldl cholesterol

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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Chicken IgY Antibody

CAT Product Name Application Type
BRD-0322MZ Chicken Anti-LDL Polyclonal IgY Anti-LDL antibody produced in chicken is suitable for indirect ELISA and WBting analysis at a dilution of 1:500, for tissue or cell staining at a dilution of 1:200 Chicken antibody

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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