CytoStream™ Rabbit Anti-LGR6 Recombinant Antibody (VS13-YC656)
CAT#: VS13-YC656
The CytoStream™ Rabbit Anti-LGR6 Recombinant Antibody is specific for human, mouse and rat LGR6. LGR6 encodes a leucine-rich repeat-containing receptor, part of GPCR family, cooperating in R-spondin-mediated Wnt signaling, acting as a stem cell marker with tumor suppressor potential. The anti-LGR6 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8556/159_F6_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8556/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8556/23734_B_8_5.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8556/23734_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8556/23734_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8556/23734_A_6_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (A549, CAPAN-2, HaCaT, HeLa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000133067-LGR6
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Specifications
- Immunogen
- Recombinant human LGR6, aa 850-967.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat LGR6
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, ICC, IF, FC
- Conjugate
- NONE
- Related Disease
- Myxedema and Eccrine Sweat Gland Neoplasm
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4) containing 150 mM NaCl and 50% Glycerol.
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Leucine Rich Repeat Containing G Protein-Coupled Receptor 6 Leucine-Rich Repeat-Containing G-Protein Coupled Receptor 6 3 Gonadotropin Receptor 3 VTS20631 3 GPCR 3
- Gene ID
- 59352
- UniProt ID
- Q9HBX8
- Long Name
- Leucine Rich Repeat Containing G Protein-Coupled Receptor 6
- Sequence Similarities
- Belongs to the G-protein coupled receptor 1 family.
- Cellular Localization
- Cell membrane
- Protein Refseq
- XP_005245461.1; XP_011508140.1; XP_011508141.1; XP_011508142.1; XP_011508143.1
- Function
- Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a marker of multipotent stem cells in the epidermis. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. May act as a tumor suppressor.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "LGR6"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1819z | Mouse Anti-LGR6 Recombinant Antibody (clone 38B7) | WB, ELISA, IF, IP | Mouse IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2049 | Hi-Affi™ Recombinant Rabbit Anti-LGR6 Monoclonal Antibody (DS2049AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0467-YJ | Mouse Anti-LGR6 Recombinant Antibody (clone Mu2C15) | ELISA, WB, ICC, FC, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0467-YJ-S(P) | Mouse Anti-LGR6 Recombinant Antibody (clone Mu2C15); scFv Fragment | ELISA, WB, ICC, FC, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0467-YJ-F(E) | Mouse Anti-LGR6 Recombinant Antibody (clone Mu2C15); Fab Fragment | ELISA, WB, ICC, FC, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1732 | Rabbit Anti-LGR6 Recombinant Antibody (clone R09-8B5) | WB, IHC-Fr, IHC-P, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC561 | Recombinant Anti-LGR6 Vesicular Antibody, EV Displayed (VS-0425-YC561) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4013 | Anti-LGR6 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4014 | Anti-Mouse LGR6 Immunohistochemistry Kit | IHC |
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