Rabbit Anti-LYN (pY396) Recombinant Antibody (clone R07-3H2) (CAT#: VS3-WK2169)
This product is a rabbit recombinant antibody that is specific to phosphorylated LYN. The target of this antibody is phospho-LYN (Tyr396). The clone R07-3H2 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200 and FC analysis at a dilution of 1/50-1/100. The molecular weight of LYN (pTyr396) observed in WB experiments using this antibody is 56 kDa.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/if_selected.jpg
Figure 2 IHC staining of human spleen
(High expression. RNA expression: 85.4 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_B_7_4_val_selected.jpg
(High expression. RNA expression: 85.4 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_B_7_4_val_selected.jpg
Figure 3 IHC staining of human cerebral cortex
(Low expression. RNA expression: 8.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_B_8_5_val_selected.jpg
(Low expression. RNA expression: 8.7 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_B_8_5_val_selected.jpg
Figure 4 Colon
(Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_9_3.jpg
(Glandular cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4492/12394_A_7_4.jpg
(Cholangiocytes
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4492/12394_A_7_4.jpg
Figure 6 Kidney
(Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_9_5.jpg
(Cells in tubules
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_9_5.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1231/4032_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (HEL, HMC-1)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000254087-LYN
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000254087-LYN
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr396 of human and mouse LYN
- Species Reactivity
- Human, Mouse
- Clone
- R07-3H2
- Applications
- WB, ICC, IF, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- JTK8; SAIDV; p53Lyn; p56Lyn
- Gene ID
- 4067
- UniProt ID
- P07948
- Long Name
- LYN proto-oncogene, Src family tyrosine kinase
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
BCR Signaling Pathway
FcεR1 Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2781z | Mouse Anti-LYN Recombinant Antibody (clone 33H4) | WB, ICC, IF, IP | Mouse IgG1 |
| MOB-1024MZ | Recombinant Mouse Anti-Human LYN Antibody (clone 3I9E8) | ELISA | Mouse antibody |
| ZG-030R | Mouse Anti-Lyn Recombinant Antibody (ZG-030R) | WB, ELISA | Mouse IgG |
| VS3-QX704 | Mouse Anti-LYN Recombinant Antibody (clone 1C8-H3-G6) | WB | Mouse IgG1 |
| VS3-QX705 | Mouse Anti-LYN Recombinant Antibody (clone 1174CT22.3.1.1) | WB | Mouse IgG1, κ |
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