Mouse Anti-MAP2 Recombinant Antibody (VS7-0425-WR331)
CAT#: VS7-0425-WR331
This product is a mouse IgG antibody that binds to human MAP2. The antibody can be used for WB, IF, and ICC assays. It cross reacts with mouse, rat, bovine, and pig.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/1895_G7_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line SH-SY5Y
Immunofluorescent staining of human cell line SH-SY5Y shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12558/1927_F10_1_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_A_7_3.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8273/24187_B_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (AF22, NTERA-2, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000078018-MAP2
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Specifications
- Immunogen
- Recombinant human MAP2 (N-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MAP2
- Species Reactivity
- Human, Mouse, Rat, Bovine, Pig
- Molecule Class
- Microtubule-Associated Protein
- Applications
- WB, IF, ICC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:1000 - 1:2000
Immunofluorescence: 1:100 - 1:500
Target
- Alternative Names
- MAP2; Microtubule-Associated Protein 2; MAP2B; MAP2C; MAP-2; MAP2A
- Gene ID
- 4133
- UniProt ID
- P11137
- Long Name
- Microtubule associated protein 2
- Cellular Localization
- Cell projection; Cytoplasm; Cytoskeleton; Microtubule
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001034627.1
- Function
- Calmodulin-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1671z | Mouse Anti-MAP2 Recombinant Antibody (clone 22D3) | WB, ELISA, ICC, IHC, IP | Mouse IgG1, κ |
| MOB-0095F | Mouse Anti-MAP2 Recombinant Antibody (clone 7D4) | IHC-P, IF | Mouse IgG |
| ZG-0267C | Mouse Anti-MAP2 Recombinant Antibody (ZG-0267C) | WB | Mouse IgG |
| ZG-0268C | Mouse Anti-MAP2 Recombinant Antibody (ZG-0268C) | WB, IF | Mouse IgG |
| VS3-WK90 | Mouse Anti-MAP2 Recombinant Antibody (clone F7-B7) | WB, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0797MZ | Chicken Anti-MAP2 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1159MZ | Chicken Anti-Microtubule Associated Protein 2 (MAP2) Polyclonal IgY | ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2130 | Hi-Affi™ Recombinant Rabbit Anti-MAP2 Monoclonal Antibody (DS2130AB) | IHC-P, IP, WB | IgG |
| MOR-0097-FY | Rabbit Anti-MAP2 Recombinant Antibody (clone AFY0068) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY161 | CytoStream™ Mouse Anti-MAP2 Recombinant Antibody (VS-0225-XY161) | FC | Mouse IgG1, kappa |
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