Rabbit Anti-MAP2K2 Recombinant Antibody (VS3-CJ916)
CAT#: VS3-CJ916
This product is a rabbit antibody that recognizes human, and mouse MAP2K2.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/640_C4_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51993/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/ihc_selected.jpg
Normal Tissue
Figure 5 Skin
Epidermal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_B_4_8.jpg
Normal Tissue
Figure 6 Lymph node
Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_8.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3835/11077_A_7_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000126934-MAP2K2
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse MAP2K2
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- CFC4; MEK2; MKK2; MAPKK2; PRKMK2
- Gene ID
- 5605
- UniProt ID
- P36507
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Cellular Localization
- Cytoplasm, Membrane
- Post Translation Modifications
- MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1 (By similarity).
(Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Protein Refseq
- NP_109587.1
- Function
- Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP2K2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2132 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (DS2132AB) | WB, FC, IP, IHC | IgG |
| MOR-4643 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (TH156DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0980-CN | Recombinant Rabbit Anti-MAP2K2 Monoclonal Antibody (CBACN-359) | WB, IF, IHC, IP, FC | Rabbit IgG |
| MOB-0103F | Mouse Anti-MAP2K2 Recombinant Antibody (clone 2C3) | WB | Mouse IgG |
| MOB-0104F | Mouse Anti-MAP2K2 Recombinant Antibody (clone 7D11) | WB | Mouse IgG |
| VS3-QX714 | Mouse Anti-MAP2K2 Recombinant Antibody (clone 9E8-7G2-5A10) | WB | Mouse IgG1 |
| VS3-QX715 | Mouse Anti-MAP2K2 Recombinant Antibody (clone 9G3-3H2-7B7) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1183 | AbPlus™ Anti-MAP2K2 Magnetic Beads (VS-0724-YC1183) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1293 | Anti-MAP2K2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4209 | Anti-Mouse MAP2K2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4208 | Anti-Human MAP2K2 Immunohistochemistry Kit | IHC |
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