Rabbit Anti-MAPK6 Recombinant Antibody (clone R04-5B8)
CAT#: VS3-FY1761
Clone R04-5B8 is a rabbit recombinant monoclonal antibody that confidently identify human MAPK6. It cross-reacts with mouse. The antibody is recommended for detection of MAPK6 protein by WB, IHC-P, IP. This MAPK6 antibody was used in western blot analysis of MAPK6 in 3T3, Hela lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-MAPK6 Antibody (VS3-FY1761) in WB.
The MAPK6 expression in 3T3, Hela lysates were detected using the anti-MAPK6 by western blot assay.
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Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30262/270_F9_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/47825/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human caudate
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5184/ihc_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5184/13370_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30262/63310_A_8_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30262/63310_A_7_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000069956-MAPK6
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Specifications
- Immunogen
- Synthetic peptides of human MAPK6
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse MAPK6
- Species Reactivity
- Human, Mouse
- Clone
- R04-5B8
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 83 kDa, while the observed molecular weight stands at 105 kDa.
- Related Disease
- Pulmonary Immaturity and Chronic Myelogenous Leukemia, Bcr-Abl1 Positive
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunoprecipitation: 1/20
Target
- Alternative Names
- Mitogen-Activated Protein Kinase 6; Extracellular Signal-Regulated Kinase 3; MAP Kinase Isoform P97; MAP Kinase 6; EC 2.7.11.24; P97-MAPK; MAPK 6; PRKM6; ERK-3
- Gene ID
- 5597
- UniProt ID
- Q16659
- Sequence Similarities
- Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.1 Publication
Ubiquitination at Met-1 leads to degradation by the proteasome pathway.
- Protein Refseq
- XP_005254594.1; XP_005254595.1; XP_011520084.1; XP_047288803.1; XP_047288804.1
- Function
- Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).
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Related Signaling Pathways
FcεR1 Signaling Pathway
TCR Signaling Pathway
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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