Mouse Anti-MCM2 Monoclonal Antibody (VS7-0425-WR369)
CAT#: VS7-0425-WR369
This product is a mouse IgG antibody that binds to human MCM2. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31450/1923_C3_26_cr5cd2b031ba569_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31495/721_D2_2_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31496/727_C2_1_selected.jpg
Normal Tissue
Figure 4 Testis
Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_4_6.jpg
Normal Tissue
Figure 5 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_B_6_4.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_8_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073111-MCM2
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Specifications
- Immunogen
- Recombinant human MCM2 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MCM2
- Species Reactivity
- Human
- Molecule Class
- Mini-Chromosome Maintenance Protein (MCM)
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Deafness, autosomal dominant, 70 (DFNA70)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- BM28; CCNL1; CDCL1; cdc19; DFNA70; D3S3194; MITOTIN
- Gene ID
- 4171
- UniProt ID
- P49736
- Long Name
- Minichromosome Maintenance Complex Component 2
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_004517.2
- Function
- DNA-binding; Helicase; Hydrolase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MCM2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0715MZ | Chicken Anti-MCM2 Polyclonal IgY | ICC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2190 | Hi-Affi™ Recombinant Rabbit Anti-MCM2 Monoclonal Antibody (DS2190AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0101F | Mouse Anti-MCM2 Recombinant Antibody (MOB-0101F) | IHC-P, IF | Mouse IgG |
| VS3-QX762 | Mouse Anti-MCM2 Recombinant Antibody (clone 2B5-H4-A9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY907 | Recombinant Rabbit Anti-MCM2 (phospho Ser108) Antibody (clone R01-7G9) | WB, ICC, IF, IP | Rabbit IgG |
| VS3-FY908 | Recombinant Rabbit Anti-MCM2 (phospho Ser40) Antibody (clone R03-1B3) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY909 | Recombinant Rabbit Anti-MCM2 (phospho Ser41) Antibody (clone R07-4C3) | WB, IHC-P, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY318 | Mouse Anti-NHP MCM2 Recombinant Antibody (clone 2B3) | WB, IHC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1319 | Anti-MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4304 | Anti-Mouse MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4303 | Anti-Human MCM2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4305 | Anti-Rat MCM2 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC711 | CytoStream™ Rabbit Anti-MCM2 Recombinant Antibody (VS13-YC711) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
| VS13-YC712 | CytoStream™ Mouse Anti-MCM2 Recombinant Antibody (VS13-YC712) | WB, IHC, IF, IP, FC | Mouse IgG1 |
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