Rabbit Anti-MCM2 (pS27) Recombinant Antibody (clone R88-9Q-1) (CAT#: VS3-WK2074)
This product is a rabbit recombinant antibody that is specific to phosphorylated MCM2. The target of this antibody is phospho-MCM2 (Ser27). The clone R88-9Q-1 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, IP analysis at a dilution of 1/50, and IHC staining at a dilution of 1/100-1/200. The molecular weight of MCM2 (pSer27) observed in WB experiments using this antibody is 125 kDa.
| Sub Cat | Clone | Species Reactivity | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS3-WK2301 | R88-9Q-1 | Human, Mouse, Rat | ||||||
| VS3-WK2238 | Human |
More Infomation
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31450/1923_C3_26_cr5cd2b031ba569_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31450/1923_C3_26_cr5cd2b031ba569_selected.jpg
Figure 2 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31495/721_D2_2_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31495/721_D2_2_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31496/727_C2_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31496/727_C2_1_selected.jpg
Figure 4 Testis
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_4_6.jpg
(Pachytene spermatocytes Staining: Low Intensity: Moderate Quantity: <25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_4_6.jpg
Figure 5 Bone marrow
(Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_B_6_4.jpg
(Hematopoietic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_B_6_4.jpg
Figure 6 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_8_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/303/2753_A_8_8.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073111-MCM2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073111-MCM2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser27 of human, mouse and rat MCM2
- Applications
- WB, IHC-P, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/100-1/200
IF: 1/50-1/200
IP: 1/50
Target
- Alternative Names
- Minichromosome Maintenance Complex Component 2; Minichromosome Maintenance Protein 2 Homolog; Nuclear Protein BM28; Mitotin; CCNL1; CDCL1; BM28; MCM2 Minichromosome Maintenance Deficient 2, Mitotin (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 2 (Mitotin); Minichromosome Maintenance Deficient 2 (Mitotin)
- Gene ID
- 4171
- UniProt ID
- P49736
- Long Name
- Minichromosome Maintenance Complex Component 2
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-537-MZ | Mouse Anti-MCM2 Recombinant Antibody (clone CRCT2.1) | WB, IHC-P | |
| MOB-1146CT | Recombinant Mouse anti-Human MCM2 Monoclonal antibody (EML554) | ICC/IF, WB | |
| MOB-0102F | Mouse Anti-MCM2 Recombinant Antibody (MOB-0102F) | IHC-P, IF | Mouse IgG |
| VS3-QX762 | Mouse Anti-MCM2 Recombinant Antibody (clone 2B5-H4-A9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS7-0425-WR368 | Mouse Anti-MCM2 Monoclonal Antibody (clone 1E7) | WB, IHC, ICC, FC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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