Rabbit Anti-MFN2 Recombinant Antibody (clone R56-8V-9)
CAT#: VS3-FY2117
Clone R56-8V-9 is a rabbit IgG recombinant monoclonal antibody that was developed to identify human MFN2 protein, a calculated weight of 86 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R56-8V-9 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200), immunofluorescence (dilution range 1:50-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79902/1987_B9_6_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30554/66797_B_4_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30554/66797_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30554/66797_A_8_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30554/66797_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30554/66797_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/30554/66797_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000116688-MFN2
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MFN2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R56-8V-9
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 86 kDa, while the observed molecular weight stands at 80kDa.
- Related Disease
- Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Mitofusin 2; Transmembrane GTPase MFN2; CPRP1; Mitochondrial Assembly Regulatory Factor; Hyperplasia Suppressor; Mitofusin-2; EC 3.6.5.-; KIAA0214; EC 3.6.5
- Gene ID
- 9927
- UniProt ID
- O95140
- Sequence Similarities
- Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. Mitofusin subfamily.
- Cellular Localization
- Membrane, Mitochondrion, Mitochondrion outer membrane
- Post Translation Modifications
- Phosphorylated by PINK1. (O95140-MFN2_HUMAN)
Ubiquitinated by non-degradative ubiquitin by PRKN, promoting mitochondrial fusion; deubiquitination by USP30 inhibits mitochondrial fusion (PubMed:23620051).
Ubiquitinated by HUWE1 when dietary stearate (C18:0) levels are low; ubiquitination inhibits mitochondrial fusion (PubMed:26214738, 30217973).
(O95140-MFN2_HUMAN)
Ubiquitination at Lys406, Lys416, and Lys420 (NX_O95140 [NX_O95140-1])
Modification sites at PhosphoSitePlus (O95140)
- Protein Refseq
- XP_005263600.1; XP_005263602.1; XP_005263605.1; XP_047292105.1; XP_047292110.1
- Function
- Plays an important role in the regulation of vascular smooth muscle cell proliferation (By similarity).
Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy).
Is required for PRKN recruitment to dysfunctional mitochondria.
Involved in the control of unfolded protein response (UPR) upon ER stress including activation of apoptosis and autophagy during ER stress (By similarity).
Acts as an upstream regulator of EIF2AK3 and suppresses EIF2AK3 activation under basal conditions (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MFN2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2223 | Hi-Affi™ Recombinant Rabbit Anti-MFN2 Monoclonal Antibody (DS2223AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2034-CN | Rabbit Anti-MFN2 Polyclonal Antibody (MRO-2034-CN) | WB, IF, FC | Rabbit IgG |
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| VS-0422-LC146 | Mouse Anti-Mfn2 Recombinant Antibody (clone mN153/5.1) | ICC, WB, IF | Mouse IgG2a |
| VS-0422-LC147 | Rabbit Anti-Mfn2 Recombinant Antibody (clone rN153/5.1) | ICC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC638 | AbPlus™ Anti-MFN2 Magnetic Beads (VS-0724-YC638) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1344 | Anti-MFN2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4374 | Anti-Mouse MFN2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4373 | Anti-Human MFN2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4375 | Anti-Rat MFN2 Immunohistochemistry Kit | IHC |
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