Mouse Anti-MITF Recombinant Antibody (clone 3A2E2) (CAT#: VS7-0425-WR385)
This product is a mouse IgG1 antibody that binds to human MITF. The antibody 3A2E2 recognizes specific amino acid domain of human MITF (AA: 1-114). It can be used for FC assay.
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Normal Tissue
RNA Expression
Figure 1 Cervix
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10295_B_7_3_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10295_B_7_3_val_selected.jpg
Figure 2 Colon
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_9_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_9_3.jpg
Figure 3 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10268_A_8_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10268_A_8_5.jpg
Figure 4 Skin
(Fibrohistiocytic cells Staining: Low Intensity: Weak Melanocytes Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_B_8_1.jpg
(Fibrohistiocytic cells Staining: Low Intensity: Weak Melanocytes Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_B_8_1.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_8_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, SK-MEL-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187098-MITF
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187098-MITF
Specifications
- Immunogen
- Recombinant human MITF (AA: 1-114) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MITF
- Species Reactivity
- Human
- Clone
- 3A2E2
- Molecule Class
- Basic Helix-Loop-Helix Leucine Zipper (BHLH-ZIP) Transcription Factor
- Applications
- FC
- Conjugate
- Unconjugated
- Related Disease
- Waardenburg syndrome 2A (WS2A); Tietz albinism-deafness syndrome (TADS); Melanoma, cutaneous malignant 8 (CMM8); Coloboma, osteopetrosis, microphthalmia, macrocephaly, albinism, and deafness (COMMAD)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MITF; Microphthalmia-Associated Transcription Factor; BHLHe32; CMM8; MI; WS2A; Class E Basic Helix-Loop-Helix Protein 32; WS2; Homolog Of Mouse Microphthalmia
- Gene ID
- 4286
- UniProt ID
- O75030
- Long Name
- Melanogenesis Associated Transcription Factor
- Cellular Localization
- Cytoplasm; Lysosome; Membrane; Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000239.1
- Function
- Activator; Developmental protein; DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1711z | Mouse Anti-MITF Recombinant Antibody (clone 28F1) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MOB-0327MZ | Recombinant Mouse Anti-Human MITF Antibody (clone E6) | IHC | Mouse antibody |
| VS3-CJ169 | Mouse Anti-MITF Recombinant Antibody (VS3-CJ169) | IHC | |
| VS3-CJ170 | Mouse Anti-MITF Recombinant Antibody (VS3-CJ170) | WB | |
| VS3-FY929 | Recombinant Rabbit Anti-MITF Antibody (clone R06-4B5) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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