Mouse Anti-MITF Recombinant Antibody (clone 8F1G5)
CAT#: VS7-0425-WR386
This product is a mouse IgG1 antibody that binds to human MITF. The antibody 8F1G5 recognizes specific amino acid domain of human MITF (AA: 1-114). It can be used for WB and FC assays.
Gene Expression
Normal Tissue
Figure 1 Cervix
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10295_B_7_3_val_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_9_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10268_A_8_5.jpg
Normal Tissue
Figure 4 Skin
Fibrohistiocytic cells Staining: Low Intensity: Weak Melanocytes Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_B_8_1.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187098-MITF
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Specifications
- Immunogen
- Recombinant human MITF (AA: 1-114) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MITF
- Species Reactivity
- Human
- Clone
- 8F1G5
- Molecule Class
- Basic Helix-Loop-Helix Leucine Zipper (BHLH-ZIP) Transcription Factor
- Applications
- WB, FC
- Conjugate
- Unconjugated
- Related Disease
- Waardenburg syndrome 2A (WS2A); Tietz albinism-deafness syndrome (TADS); Melanoma, cutaneous malignant 8 (CMM8); Coloboma, osteopetrosis, microphthalmia, macrocephaly, albinism, and deafness (COMMAD)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MITF; Microphthalmia-Associated Transcription Factor; BHLHe32; CMM8; MI; WS2A; Class E Basic Helix-Loop-Helix Protein 32; WS2; Homolog Of Mouse Microphthalmia
- Gene ID
- 4286
- UniProt ID
- O75030
- Long Name
- Melanogenesis Associated Transcription Factor
- Cellular Localization
- Cytoplasm; Lysosome; Membrane; Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000239.1
- Function
- Activator; Developmental protein; DNA-binding
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MITF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0362MZ | Chicken Anti-MITF Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2236 | Hi-Affi™ Recombinant Rabbit Anti-MITF Monoclonal Antibody (DS2236AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1002-CN | Recombinant Rabbit Anti-MITF Monoclonal Antibody (JF100-01) | WB, IF, FC | Rabbit IgG |
| VS3-CJ169 | Mouse Anti-MITF Recombinant Antibody (VS3-CJ169) | IHC | |
| VS3-CJ170 | Mouse Anti-MITF Recombinant Antibody (VS3-CJ170) | WB | |
| VS3-FY929 | Recombinant Rabbit Anti-MITF Antibody (clone R06-4B5) | WB | Rabbit IgG |
| VS7-0425-WR385 | Mouse Anti-MITF Recombinant Antibody (clone 3A2E2) | FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY187 | AbPlus™ Anti-MITF Magnetic Beads (C5) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC729 | CytoStream™ Rabbit Anti-MITF Recombinant Antibody (VS13-YC729) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4402 | Anti-MITF Immunohistochemistry Kit | IHC |
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