Mouse Anti-MITF Recombinant Antibody (VS3-CJ170)
CAT#: VS3-CJ170
This product is a mouse antibody that recognizes MITF.
Gene Expression
Normal Tissue
Figure 1 Cervix
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10295_B_7_3_val_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_9_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10268_A_8_5.jpg
Normal Tissue
Figure 4 Skin
Fibrohistiocytic cells Staining: Low Intensity: Weak Melanocytes Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_B_8_1.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187098-MITF
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Specifications
- Immunogen
- Recombinant protein of human MITF
- Host Species
- Mouse
- Specificity
- Human, Mouse and Rat MITF
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS
- Preservative
- 0.09% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:2000).
Target
- Alternative Names
- MI; WS2; CMM8; WS2A; COMMAD; MITF-A; bHLHe32
- Gene ID
- 4286
- UniProt ID
- O75030
- Sequence Similarities
- Belongs to the MiT/TFE family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter (PubMed:10587587).
Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (PubMed:10673502).
Phosphorylated in response to blue light (415nm) (PubMed:28842328).
Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation.
- Protein Refseq
- NP_000239.1; NP_001171896.1; NP_006713.1
- Function
- Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MITF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0362MZ | Chicken Anti-MITF Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2236 | Hi-Affi™ Recombinant Rabbit Anti-MITF Monoclonal Antibody (DS2236AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1002-CN | Recombinant Rabbit Anti-MITF Monoclonal Antibody (JF100-01) | WB, IF, FC | Rabbit IgG |
| ZG-0774F | Mouse Anti-MITF Recombinant Antibody (clone C5) | WB, IP, IHC, GS | Mouse IgG1 |
| ZG-0782F | Mouse Anti-MITF Antibody (clone C5/D5 Cocktail) | IHC, IP, WB, GS | Mouse IgG1 |
| ZG-0788F | Mouse Anti-MITF Recombinant Antibody (clone D5) | IHC, GS, WB, IP | Mouse IgG1 |
| VS3-WK417 | Rabbit Anti-MITF Recombinant Antibody (clone JF100-01) | WB, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY187 | AbPlus™ Anti-MITF Magnetic Beads (C5) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC729 | CytoStream™ Rabbit Anti-MITF Recombinant Antibody (VS13-YC729) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4402 | Anti-MITF Immunohistochemistry Kit | IHC |
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