Mouse Anti-MKI67 Monoclonal Antibody (VS7-0425-WR389)
CAT#: VS7-0425-WR389
This product is a mouse IgG antibody that binds to human MKI67. The antibody recognizes specific amino acid domain of human MKI67 aa (CEDLAGFKELFQTPG). It can be used for WB, IHC-P, IF, and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/13_B8_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/952_H5_3_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/30_B1_1_red_green.jpg
Normal Tissue
Figure 4 Colon
Goblet cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Mucosal lymphoid cells
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_7_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_6_6.jpg
Normal Tissue
Figure 6 Skin
Cells in basal layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Cells in spinous layer
Staining: Medium
Intensity: Strong
Quantity: <25%
Hair follicles
Staining: High
Intensity: Strong
Quantity: 75%-25%
Lymphocytes
Staining: High
Intensity: Strong
Sebaceous glands
Staining: Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_B_7_1.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/58/155454_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000148773-MKI67
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Specifications
- Immunogen
- Recombinant human MKI6 7aa (CEDLAGFKELFQTPG) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human MKI67
- Species Reactivity
- Human
- Molecule Class
- Nuclear Protein
- Applications
- WB, IHC-P, IF, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- KIA; MIB-; MIB-1; PPP1R105
- Gene ID
- 4288
- UniProt ID
- P46013
- Long Name
- Marker Of Proliferation Ki-67
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001139438.1
- Function
- DNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MKI67"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2237 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (DS2237AB) | ELISA, WB, IHC | IgG |
| MOR-4638 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (TH151DS) | IF, ICC, FC, ChIP | IgG |
| MOR-0054-FY | Rabbit Anti-MKI67 Recombinant Antibody (clone AFY0025) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0899-CN | Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (CBACN-337) | WB, IHC, FC | Rabbit IgG |
| MRO-1993-CN | Rabbit Anti-MKI67 Polyclonal Antibody (MRO-1993-CN) | WB, IF, IHC, FC | Rabbit IgG |
| MRO-1994-CN | Rabbit Anti-MKI67 Polyclonal Antibody (MRO-1994-CN) | WB, IHC | Rabbit IgG |
| VS3-CJ39 | Mouse Anti-MKi67 Recombinant Antibody (VS3-CJ39) | WB, ELISA | Mouse IgG |
| VS3-XY1073 | Mouse Anti-MKI67 Recombinant Antibody (clone 4A1) | ELISA, WB, IHC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2357LY-F(E) | Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); Fab Fragment | IHC, ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2357LY-S(P) | Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); scfv Fragment | IHC, ELISA, WB | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC730 | CytoStream™ Rabbit Anti-MKI67 Recombinant Antibody (VS13-YC730) | IHC-P, IHC-Fr, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4403 | Anti-MKI67 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4404 | Anti-Mouse MKI67 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4405 | Anti-Rabbit MKI67 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4406 | Anti-Canine MKI67 Immunohistochemistry Kit | IHC |
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