Mouse Anti-MKI67 Recombinant Antibody (clone ABT-KI67)
CAT#: VS3-CJ971
This product is a mouse antibody that recognizes human MKI67.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1164/13_B8_2_selected.jpg
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Specifications
- Immunogen
- Synthesized peptide derived from human Ki-67
- Host Species
- Mouse
- Type
- Mouse IgG1, κ
- Specificity
- Human MKI67
- Species Reactivity
- Human
- Clone
- ABT-KI67
- Applications
- IF, IHC-P
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Immunofluorescence (1:50-1:200), Immunohistochemistry-Parraffin (1:100-1:500).
Target
- Alternative Names
- KIA; MIB-; MIB-1; PPP1R105
- Gene ID
- 4288
- UniProt ID
- P46013
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604).
Hyperphosphorylated form does not bind DNA.
- Protein Refseq
- NP_001139438.1; NP_002408.3
- Function
- Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226).
Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226).
Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).
Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551).
Does not contribute to the internal structure of mitotic chromosomes (By similarity).
May play a role in chromatin organization (PubMed:24867636).
It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MKI67"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1072z | Mouse Anti-MKI67 Recombinant Antibody (clone 26F12) | WB, ELISA, IHC | Mouse IgG1 |
| MRO-0899-CN | Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (CBACN-337) | WB, IHC, FC | Rabbit IgG |
| ZG-0599F | Mouse Anti-MKI67 Recombinant Antibody (clone MIB-1) | IHC-Fr, IHC-P | Mouse IgG1 |
| VS3-CJ972 | Mouse Anti-MKI67 Recombinant Antibody (clone PT2017) | IF, IHC-P | Mouse IgG |
| VS3-CJ973 | Rabbit Anti-MKI67 Recombinant Antibody (VS3-CJ973) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2237 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (DS2237AB) | ELISA, WB, IHC | IgG |
| MOR-4638 | Hi-Affi™ Recombinant Rabbit Anti-MKI67 Monoclonal Antibody (TH151DS) | IF, ICC, FC, ChIP | IgG |
| MOR-0054-FY | Rabbit Anti-MKI67 Recombinant Antibody (clone AFY0025) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2357LY-F(E) | Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); Fab Fragment | IHC, ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2357LY-S(P) | Mouse Anti-MKI67 Recombinant Antibody (clone 73043-24E8); scfv Fragment | IHC, ELISA, WB | Mouse scfv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC730 | CytoStream™ Rabbit Anti-MKI67 Recombinant Antibody (VS13-YC730) | IHC-P, IHC-Fr, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4403 | Anti-MKI67 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4404 | Anti-Mouse MKI67 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4405 | Anti-Rabbit MKI67 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4406 | Anti-Canine MKI67 Immunohistochemistry Kit | IHC |
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