SmartAb™ Recombinant Anti-MMP2 pH-dependent Antibody (Clone 3G11)
CAT#: VS-0825-YC255
The anti-MMP2 pH-dependent antibody (clone 3G11) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human MMP2. By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for MMP2 under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from MMP2 at pH 6.0. This pH-sensitive antibody retains its binding affinity on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. Human MMP2, also known as Gelatinase A, is a protease that degrades gelatin and other components of the extracellular matrix. It is involved in tissue remodeling and wound healing and is a key mediator of cancer invasion and metastasis.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_B_5_3_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/7518_A_9_4.jpg
Normal Tissue
Figure 7 Gallbladder
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/7518_A_4_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/7518_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, hTERT-RPE1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087245-MMP2
❮
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Specifications
- Immunogen
- Recombinant human type IV collagenase.
- Host Species
- Human
- Type
- Human IgG1 kappa
- Specificity
- Human MMP2
- Species Reactivity
- Human
- Clone
- 3G11
- Applications
- ELISA
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
- Alternative Names
- Matrix Metallopeptidase 2; Matrix Metalloproteinase-2; EC 3.4.24.24; CLG4A; MMP-2; TBE-1; Matrix Metalloproteinase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metallopeptidase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metalloproteinase-II; 72 KDa Type IV Collagenase
- Gene ID
- 4313
- UniProt ID
- P08253
- Long Name
- Matrix Metallopeptidase 2
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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