Anti-Mouse VIM Immunohistochemistry Kit (CAT#: VS-0525-XY7852)
The VIM IHC Kit delivers dependable staining for Vimentin (VIM), a key marker of mesenchymal cells. It includes all required reagents for use on both paraffin-embedded and frozen sections and supports multi-species analysis. This kit is widely used in studies of epithelial-mesenchymal transition (EMT), tumor progression, and wound healing.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line ASC TERT1
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
(Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg

Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg

Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg

Figure 4 Cerebral cortex
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg

Figure 5 Colon
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:
High
Intensity: Strong
Mucosal lymphoid cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
(Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:
High
Intensity: Strong
Mucosal lymphoid cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg

Figure 6 Kidney
(Cells in glomeruli
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
(Cells in glomeruli
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg

Figure 7 Testis
(Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:
High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
(Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:
High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg

Figure 8 Breast
(Adipocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Myoepithelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
(Adipocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Myoepithelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg

Figure 9 Lymph node
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg

Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000026025-VIM
(Cell lines ordered by descending RNA expression order.)
* https://v21.proteinatlas.org/ENSG00000026025-VIM

Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM
Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- VIM
- Primary Antibody
- Rat Anti-VIM Antibody
- Secondary Antibody
- Goat anti-Rat Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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IHC Kits & Antibodies
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1153z | Mouse Anti-VIM Recombinant Antibody (clone 28A3) | WB, ELISA, IHC, IP | Mouse IgG1, κ |
MRO-1583-CN | Mouse Anti-VIM Recombinant Antibody (clone A6-C1) | WB, IF, IHC, FC | Mouse IgG2b |
MRO-1584-CN | Rabbit Anti-VIM Recombinant Antibody (clone CBACN-580) | WB, IF, IHC, IP, FC | Rabbit IgG |
MRO-1585-CN | Mouse Anti-VIM Recombinant Antibody (clone D4-B11) | WB, IF, IHC, FC | Mouse IgG1 |
VS3-XY1589 | Mouse Anti-VIM Recombinant Antibody (clone B4-B11) | WB, ICC, IHC, FC | Mouse IgG1 |
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