Anti-Mouse VIM Immunohistochemistry Kit

Name: Anti-Mouse VIM Immunohistochemistry Kit
SKU: VS-0525-XY7852
Rating: 5 (1 reviews)

CAT#: VS-0525-XY7852

The VIM IHC Kit delivers dependable staining for Vimentin (VIM), a key marker of mesenchymal cells. It includes all required reagents for use on both paraffin-embedded and frozen sections and supports multi-species analysis. This kit is widely used in studies of epithelial-mesenchymal transition (EMT), tumor progression, and wound healing.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line ASC TERT1

Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg

Subcellular Location Figure 2 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg

Subcellular Location Figure 3 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg

Normal Tissue Figure 4 Cerebral cortex

Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous

Neuropil
Staining:

Medium

Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg

Normal Tissue Figure 5 Colon

Endothelial cells
Staining:

High

Intensity: Strong
Quantity:>75%

Fibroblasts
Staining:

High

Intensity: Strong

Mucosal lymphoid cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg

Normal Tissue Figure 6 Kidney

Cells in glomeruli
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:

Medium

Intensity: Strong
Quantity: <25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg

Normal Tissue Figure 7 Testis

Leydig cells
Staining:

High

Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:

High

Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:

High

Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:

High

Intensity: Strong
Quantity:>75%

Sertoli cells
Staining:

High

Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:

High

Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg

Normal Tissue Figure 8 Breast

Adipocytes
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear

Myoepithelial cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg

Normal Tissue Figure 9 Lymph node

Germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:

High

Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)

Cell lines ordered by descending RNA expression order.

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM

❮

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Specifications

Application
IHC

Size
50 Tests

Species Reactivity
Human, Mouse, Rat

Target
VIM

Primary Antibody
Rat Anti-VIM Antibody

Secondary Antibody
Goat anti-Rat Antibody, HRP

Sample Type
FFPE tissue; Frozen section tissue

Kit Storage
All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.

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Downloadable Resources

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Datasheet

Datasheet - VS-0525-XY7852.pdf
IHC Kits-FFPE Protocol.pdf
IHC Kits-FS Protocol.pdf

MSDS

MSDS - VS-0525-XY7852.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunohistochemistry (IHC)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

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