AbPlus™ Anti-VIM Magnetic Beads (NKI-M9)
CAT#: VS-0424-XY274
The AbPlus Anti-VIM Magnetic Beads (NKI-M9) is an innovative affinity resin which is bound with anti-VIM specific antibody (NKI-M9 (former PAF2a)). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of VIM protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:
High
Intensity: Strong
Mucosal lymphoid cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:
High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
Normal Tissue
Figure 8 Breast
Adipocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Myoepithelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM
❮
❯
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-VIM specific antibody (NKI-M9 (former PAF2a))
- Target
- VIM
- Target Species
- Human
- Antibody Clone
- NKI-M9 (former PAF2a)
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.
- Alternative Names
- Vimentin
- Gene ID
- 7431
- UniProt ID
- P08670
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "VIM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-353CQ | Anti-Human VIM Recombinant Antibody (HoAKs-1) | ELISA | |
| TAB-354CQ | Anti-Human VIM Recombinant Antibody (2F6-1) | IF | |
| TAB-353CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (HoAKs-1) | ELISA, WB | |
| TAB-354CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (2F6-1) | ELISA, WB | |
| TAB-355CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (3F9-1) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0741MZ | Chicken Anti-VIM Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3838 | Rabbit Anti-VIM Recombinant Antibody (clone DS3838AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-F(E) | Human Anti-VIM Recombinant Antibody Fab Fragment (HPAB-2717LY-F(E)) | FC, WB, Block, ELISA | Humanized Fab |
| HPAB-0349-YJ-F(E) | Mouse Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0349-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse Fab |
| HPAB-0350-YJ-F(E) | Human Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0350-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-S(P) | Human Anti-VIM Recombinant Antibody scFv Fragment (HPAB-2717LY-S(P)) | FC, WB, Block, ELISA | Humanized scfv |
| HPAB-0349-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0349-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| HPAB-0350-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0350-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF038 | DRB1*04:01/Human cit vimentin (GVYAT[cit]SSAV[cit]L[cit]SSVPGVR) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-1066F | Mouse Anti-VIM Recombinant Antibody (clone NKI-M9 (former PAF2a)) | IF, IHC-Fr, IP | Mouse IgG2a |
| ZG-1102F | Mouse Anti-VIM Recombinant Antibody (clone RV204) | IHC, ICC, IB | Mouse IgG1 |
| ZG-1103F | Rabbit Anti-VIM Recombinant Antibody (clone RV205) | IHC, ICC, IB | Rabbit IgG |
| ZG-1113F | Mouse Anti-VIM Recombinant Antibody (clone V9) | FC, ICC, IHC-Fr, IHC-P, WB | Mouse IgG1 |
| VS3-QX1236 | Mouse Anti-VIM Recombinant Antibody (clone 4F8-A5-H5) | WB, IF, IP | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1237 | Mouse Anti-VIM Recombinant Antibody (clone 280CT3.4.6) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY193 | Mouse Anti-VIM (clone HoAKs-1) scFv-Fc Chimera | WB, IHC, FC | Mouse IgG1, scFv-Fc |
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