Mouse Anti-MPO Recombinant Antibody (clone 9B12G7)
CAT#: VS7-0425-WR450
This product is a mouse IgG1 antibody that binds to human MPO. The antibody 9B12G7 recognizes specific amino acid domain of MPO (aa1-193). It can be used for WB and ELISA assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1487_B11_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1403_F7_1_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/1398_F7_1_red_green.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/61464/145726_B_6_4.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/21147/146573_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Group enriched (HL-60, NB-4)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000005381-MPO
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Specifications
- Immunogen
- Recombinant MPO (aa1-193) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MPO
- Species Reactivity
- Human
- Clone
- 9B12G7
- Molecule Class
- Heme-Containing Peroxidase Enzyme
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Myeloperoxidase deficiency (MPOD)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MPO; myeloperoxidase
- Gene ID
- 4353
- UniProt ID
- P05164
- Long Name
- Myeloperoxidase
- Cellular Localization
- Lysosome
- Post Translation Modifications
- Disulfide bond; Glycoprotein; Oxidation
- Protein Refseq
- NP_000241.1
- Function
- Oxidoreductase; Peroxidase
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
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Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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