Mouse Anti-MSH6 Recombinant Antibody (clone 3E1)
CAT#: VS7-0425-WR421
This product is a mouse IgG2a antibody that binds to human MSH6. The antibody 3E1 can be used for WB, IHC, ICC, and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/9091/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, golgi apparatus & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/290_E8_2_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/28376/249_E8_1_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_B_8_5.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_7_3.jpg
Normal Tissue
Figure 8 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_4.jpg
Normal Tissue
Figure 9 Kidney
Cells in glomeruli
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_8_5.jpg
Normal Tissue
Figure 10 Testis
Elongated or late spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_6_6.jpg
Normal Tissue
Figure 11 Ovary
Ovarian stroma cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_4_7.jpg
Normal Tissue
Figure 12 Lymph node
Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70870/151813_A_9_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000116062-MSH6
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Specifications
- Immunogen
- Recombinant human MSH6 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human MSH6
- Species Reactivity
- Human
- Clone
- 3E1
- Molecule Class
- Dna Mismatch Repair Protein
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Lynch syndrome 5 (LYNCH5); Endometrial cancer (ENDMC); Mismatch repair cancer syndrome 3 (MMRCS3); Colorectal cancer (CRC)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MSH6; mutS homolog 6; GTBP; HSAP; p160; GTMBP; HNPCC5; DNA mismatch repair protein Msh6; G/T mismatch-binding protein; mutS-alpha 160 kDa subunit; sperm-associated protein
- Gene ID
- 2956
- UniProt ID
- P52701
- Long Name
- MutS Homolog 6
- Cellular Localization
- Chromosome; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_000170.1
- Function
- DNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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