Mouse Anti-MYCN Recombinant Antibody (clone 2A4E9)
CAT#: VS7-0425-WR449
This product is a mouse IgG1 antibody that binds to human MYCN. The antibody 2A4E9 recognizes specific amino acid domain of human MYCN (AA: 337-464). It can be used for IHC and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoli.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/57420/1610_C12_1_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Cell line enhanced (AF22, BEWO, HMC-1, NTERA-2, RH-30, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000134323-MYCN
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Specifications
- Immunogen
- Recombinant human MYCN (AA: 337-464) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human MYCN
- Species Reactivity
- Human
- Clone
- 2A4E9
- Molecule Class
- Helix-Loop-Helix Transcription Factor
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Feingold syndrome 1 (FGLDS1); Megalencephaly-polydactyly syndrome (MPAPA)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- MYCN; ODED; Pp65/67; N-Myc Proto-Oncogene Protein; V-Myc Avian Myelocytomatosis Viral Oncogene Neuroblastoma Derived Homolog; Class E Basic Helix-Loop-Helix Protein 37; Oncogene NMYC; NMYC; Neuroblastoma-Derived V-Myc Avian Myelocytomatosis Viral Related
- Gene ID
- 4613
- UniProt ID
- P04198
- Long Name
- MYCN Proto-Oncogene, BHLH Transcription Factor
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001280157.1
- Function
- Activator; DNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MYCN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3997z | Mouse Anti-MYCN Recombinant Antibody (clone 18H10) | WB, IP, IF | Mouse IgG2a |
| MOB-2256CT | Recombinant Mouse anti-Human MYCN Monoclonal antibody (3H4) | WB | |
| VS3-FY2814 | Mouse Anti-MYCN Recombinant Antibody (VS3-FY2814) | IHC, FC, ELISA | Mouse IgG |
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