Mouse Anti-NANOG Recombinant Antibody (clone 8A1D11) (CAT#: ZG-0235U)

Figure 1 Mouse Anti-NANOG Antibody (ZG-0235U) in WB

Positive WB detected in: Rat brain tissue, Mouse brain tissue All lanes: NANOG antibody at 1: 500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 33 kDa
Observed band size: 46, 42 kDa

Figure 2 Mouse Anti-NANOG Antibody (ZG-0235U) in IHC

Immunocytochemistry analysis of ZG-0235U diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Figure 3 Mouse Anti-NANOG Antibody (ZG-0235U) in IF

Immunofluorescence staining of Ntera-2 cells with ZG-0235U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).

Figure 4 Mouse Anti-NANOG Antibody (ZG-0235U) in FC

Overlay histogram showing Hela cells stained with ZG-0235U (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.