Mouse Anti-NANOG Recombinant Antibody (clone 8A1D11) (CAT#: ZG-0235U)
Mouse anti-NANOG antibody (clone 8A1D11) displayed high specificity against the NANOG protein of Human, Mouse and Rat.
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WB
Figure 1 Mouse Anti-NANOG Antibody (ZG-0235U) in WB
Positive WB detected in: Rat brain tissue, Mouse brain tissue All lanes: NANOG antibody at 1: 500
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 33 kDa
Observed band size: 46, 42 kDa
IHC
Figure 2 Mouse Anti-NANOG Antibody (ZG-0235U) in IHC
Immunocytochemistry analysis of ZG-0235U diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Mouse Anti-NANOG Antibody (ZG-0235U) in IF
Immunofluorescence staining of Ntera-2 cells with ZG-0235U at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 4 Mouse Anti-NANOG Antibody (ZG-0235U) in FC
Overlay histogram showing Hela cells stained with ZG-0235U (red line) at 1:250. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- Recombinant Human Homeobox protein NANOG protein (1-305AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NANOG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- 8A1D11
- Applications
- ELISA, WB, ICC, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Teratocarcinoma and Germ Cell And Embryonal Cancer.
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry. Specific conditions should be optimized by the end user.
WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200
Target
- Alternative Names
- Nanog homeobox; homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
- Gene ID
- 79923
- UniProt ID
- Q9H9S0
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| MOR-2363 | Hi-Affi™ Recombinant Rabbit Anti-NANOG Monoclonal Antibody (DS2363AB) | IHC-P | IgG |
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For Research Use Only. Not For Clinical Use.
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