Rabbit Anti-NDC80 Recombinant Antibody (clone R09-3C6)
CAT#: VS3-XY3444
The rabbit recombinant antibody specifically binds to human NDC80 (NDC80 kinetochore complex component), which plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. It also has cross-reactivity against mouse and rat NDC80. It has been detected using Western Blot and Immunoprecipitation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/66330/1754_H11_32_selected.jpg
RNA Expression
Figure 2 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000080986-NDC80
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NDC80
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R09-3C6
- Applications
- WB, IP
- Related Disease
- Endometrial Adenosquamous Carcinoma; Endometrial Adenocarcinoma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IP: 1/20
Target
- Alternative Names
- HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
- Gene ID
- 10403
- UniProt ID
- O14777
- Long Name
- NDC80 kinetochore complex component
- Cellular Localization
- Nucleus, Chromosome, centromere, kinetochore
- Post Translation Modifications
- Phosphorylation begins in S phase of the cell cycle and peaks in mitosis.
Phosphorylated by NEK2.
Also phosphorylated by AURKA and AURKB.
(O14777-NDC80_HUMAN)
Acetylated at Lys-53 and Lys-59 by KAT5 during mitosis, promoting robust kinetochore-microtubule attachment (PubMed:30409912).
Deacetylated by SIRT1.
- Protein Refseq
- NP_006092.1
- Function
- Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity.
Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.
The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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- Product Name
Recommended Dilution Buffer
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Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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