Mouse Anti-NDUFAF1 Recombinant Antibody (ND1080)
CAT#: VS-0326-YC1080
The anti-NDUFAF1 recombinant antibody (ND1080) reacts with human NDUFAF1. NDUFAF1 is a molecular chaperone essential for the assembly of Mitochondrial Complex I. It localizes to the inner mitochondrial membrane and coordinates the integration of various subunits into the incipient complex. NDUFAF1 signaling ensures the functional integrity of the electron transport chain; mutations lead to severe mitochondrial diseases like Leigh syndrome. The anti-NDUFAF1 antibody (ND1080) has been tested in Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blot (WB).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/565_B11_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/39933/95296_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137806-NDUFAF1
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NDUFAF1
- Species Reactivity
- Human
- Clone
- ND1080
- Applications
- IHC, IP, WB
- Conjugate
- The unconjugated, Biotin and FITC formats are available.
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB (1/500-1/1000), IHC (1/100-1/200), IP (1/100-1/200)
Target
- Alternative Names
- NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 1; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 1; CIA30; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, Assembly Factor 1; NADH-Ubiquinone Oxidoreductase 1 Alpha Subcomplex, Assembly Factor 1; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 1; Complex I Intermediate-Associated Protein 30, Mitochondrial; CGI-65; CGI65;
- Gene ID
- 51103
- UniProt ID
- Q9Y375
- Long Name
- NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 1
- Post Translation Modifications
- Unmodified
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NDUFAF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2399 | Hi-Affi™ Recombinant Rabbit Anti-NDUFAF1 Monoclonal Antibody (DS2399AB) | FC, ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK373 | Rabbit Anti-NDUFAF1 Recombinant Antibody (clone JE47-18) | WB, ICC, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-FY1787 | Rabbit Anti-NDUFAF1 Recombinant Antibody (clone R04-2J6) | WB, IHC-Fr, IHC-P, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1471 | Anti-NDUFAF1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4736 | Anti-Human NDUFAF1 Immunohistochemistry Kit | IHC |
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