Rabbit Anti-NEFM Recombinant Antibody (VS-0223-WK52)
CAT#: VS-0223-WK52
The anti-NEFM antibody was generated by immunizing Rabbit. The immunogen is recombinant protein corresponding to amino acids 761 to 846 from rat Neurofilament M. This antibody has been tested for use in Western Blot (1/1000), Immunocytochemistry (1/500), Immunohistochemistry (1/500) and Immunohistochemistry-Paraffin (1/1000).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22845/1729_F9_1_selected.jpg
Normal Tissue
Figure 2 High expression in cerebral cortex
Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22845/53155_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22845/53155_A_7_3.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (HEK93)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000104722-NEFM
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Specifications
- Immunogen
- Recombinant protein corresponding to AA 761 to 846 from rat Neurofilament M
- Host Species
- Rabbit
- Derivation
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Rat NEFM
- Species Reactivity
- Mouse, Rat
- Applications
- WB, ICC, IHC, IHC-P
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Lyophilized powder
- Storage
- Aliquot and store at -20°C to -80°C for 1 year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:1000), Immunocytochemistry (1:500), Immunohistochemistry (1:500) and Immunohistochemistry-Paraffin (1:1000).
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "NEFM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2305z | Mouse Anti-NEFM Recombinant Antibody (clone 39A11) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| MOB-2459CT | Recombinant Mouse anti-Cow NEF3 Monoclonal antibody (3H11) | IP, IHC-P, ELISA, WB | |
| MRO-1073-CN | Recombinant Rabbit Anti-NEFM Monoclonal Antibody (JM11-20) | WB, IF, IHC, IP | Rabbit IgG |
| ZG-1090F | Mouse Anti-NEFM Recombinant Antibody (clone RNF403) | IHC-Fr, IHC-P, WB, IF | Mouse IgG1 |
| ZG-1093F | Mouse Anti-NEFM Recombinant Antibody (clone RNF406) | IHC-Fr, IHC-P, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0816MZ | Chicken Anti-NEFM Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| BRD-1155MZ | Chicken Anti-Neurofilament Medium Polyclonal IgY | WB, ICC | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2412 | Hi-Affi™ Recombinant Rabbit Anti-NEFM Monoclonal Antibody (DS2412AB) | ICC, IF, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY339 | Mouse Anti-NHP NEFM Recombinant Antibody (clone RNF 406) | WB, IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4781 | Anti-NEFM Immunohistochemistry Kit | IHC | |
| VS-0525-XY4782 | Anti-Mouse NEFM Immunohistochemistry Kit | IHC | |
| VS-0525-XY4783 | Anti-Rat NEFM Immunohistochemistry Kit | IHC |
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