Mouse Anti-NES Recombinant Antibody (clone 10C2)
CAT#: ZG-0550F
This product is a mouse antibody that recognizes NES.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6286/1401_E3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/1783_C4_5_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5889/613_B1_1_selected.jpg
Normal Tissue
Figure 4 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7007/20723_B_8_4_val_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_8_5.jpg
Normal Tissue
Figure 8 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132688-NES
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Specifications
- Immunogen
- Recombinant 150 aa peptide of C-terminal human nestin, coupled with GST
- Host Species
- Mouse
- Type
- Mouse IgG1
- Species Reactivity
- Human
- Clone
- 10C2
- Applications
- Western Blot, Immunohistochemistry, Immunoprecipitation
Product Property
- Format
- Liquid
- Concentration
- 0.1 mg/mL
- Buffer
- PBS pH 7.3, 0.5% BSA
- Preservative
- 0.09% Sodium azide
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1: 100-1: 1000
Immunohistochemistry: 1: 50-1: 200
ImmunoprecImmunoprecipitationitation: 1: 50-1: 100 (for 100 µg/ml cell lysate)
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "Clone 10C2"
- CAT
- Product Name
See other products for "NES"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1043MZ | Recombinant Mouse Anti-Human NES Antibody (clone 3D2.4B22) | FC, IP WB | Mouse antibody |
| MRO-2064-CN | Rabbit Anti-NES Polyclonal Antibody (MRO-2064-CN) | IHC | Rabbit IgG |
| ZG-0156U | Mouse Anti-NES Recombinant Antibody (clone 2C2F7) | ELISA, WB, IHC, IF, FC | Mouse IgG1 |
| VS13-YC785 | Rabbit Anti-NES Recombinant Antibody (VS13-YC785) | WB, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR464 | Mouse Anti-NES Recombinant Antibody (clone 1A9G12) | WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0721MZ | Chicken Anti-Nes Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2422 | Hi-Affi™ Recombinant Rabbit Anti-NES Monoclonal Antibody (DS2422AB) | IHC-P, WB | IgG |
| MOR-0108-FY | Rabbit Anti-NES Recombinant Antibody (clone AFY0079) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC623 | AbPlus™ Anti-NES Magnetic Beads (VS-0724-YC623) | IP, Protein Purification | |
| VS-0724-YC624 | AbPlus™ Anti-NES Magnetic Beads (VS-0724-YC624) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC75 | Mouse Anti-NES Recombinant Antibody (VS-0924-YC75) - Cancer Stem Cell Marker | IHC, WB, IF | Mouse IgG1 |
| VS-0924-YC78 | Rabbit Anti-NES Antibody (VS-0924-YC78) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4792 | Anti-NES Immunohistochemistry Kit | IHC | |
| VS-0525-XY4793 | Anti-Mouse NES Immunohistochemistry Kit | IHC |
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