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Mouse Anti-NES Recombinant Antibody (clone 10C2) (CAT#: ZG-0550F)

This product is a mouse antibody that recognizes NES.


See more Engineered Antibody products for NES
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Subcellular Location
Figure 1 IF staining of human cell line RH-30
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/6286/1401_E3_2_selected.jpg
Figure 2 IF staining of human cell line AF22
Figure 2 IF staining of human cell line AF22
(Immunofluorescent staining of human cell line AF22 shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/1783_C4_5_selected.jpg
Figure 3 IF staining of human cell line U-2 OS
Figure 3 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/5889/613_B1_1_selected.jpg
Normal Tissue
Figure 4 Spleen
Figure 4 Spleen
(High expression in spleen)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7007/20723_B_8_4_val_selected.jpg
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_7_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_7_3.jpg
Figure 7 Kidney
Figure 7 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_8_5.jpg
Figure 8 Heart muscle
Figure 8 Heart muscle
(Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132688-NES

Specifications

  • Immunogen
  • Recombinant 150 aa peptide of C-terminal human nestin, coupled with GST
  • Host Species
  • Mouse
  • Type
  • Mouse IgG1
  • Species Reactivity
  • Human
  • Clone
  • 10C2
  • Applications
  • Western Blot, Immunohistochemistry, Immunoprecipitation

Product Property

  • Format
  • Liquid
  • Concentration
  • 0.1 mg/mL
  • Buffer
  • PBS pH 7.3, 0.5% BSA
  • Preservative
  • 0.09% Sodium azide
  • Storage
  • Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1: 100-1: 1000
    Immunohistochemistry: 1: 50-1: 200
    ImmunoprecImmunoprecipitationitation: 1: 50-1: 100 (for 100 µg/ml cell lysate)

Target

  • Alternative Names
  • Nestin; Nbla00170

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "Clone 10C2"

See other products for "NES"

Recombinant Antibody

CAT Product Name Application Type
MOB-1043MZ Recombinant Mouse Anti-Human NES Antibody (clone 3D2.4B22) FC, IP WB Mouse antibody
MRO-1075-CN Recombinant Rabbit Anti-NES Monoclonal Antibody (CBACN-397) IF, IHC Rabbit IgG
MRO-2064-CN Rabbit Anti-NES Polyclonal Antibody (MRO-2064-CN) IHC Rabbit IgG
MRO-2065-CN Rabbit Anti-NES Polyclonal Antibody (MRO-2065-CN) IF, IHC, FC, WB Rabbit IgG
VS3-CJ983 Rabbit Anti-NES Recombinant Antibody (VS3-CJ983) ICC, IF, IHC Rabbit IgG

Chicken IgY Antibody

CAT Product Name Application Type
BRD-0721MZ Chicken Anti-Nes Polyclonal IgY ICC/IF, IHC, WB Chicken antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2422 Hi-Affi™ Recombinant Rabbit Anti-NES Monoclonal Antibody (DS2422AB) IHC-P, WB IgG
MOR-0108-FY Rabbit Anti-NES Recombinant Antibody (clone AFY0079) ICC, IHC, WB Rabbit IgG

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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