Mouse Anti-NHP CCNB2 Recombinant Antibody (clone X29.2)
CAT#: VS-1024-XY147
This mouse-derived monoclonal antibody targets CCNB2 with proven reactivity to human, mouse, and non-human primate samples. Designed with precision, it is ideal for a range of applications including Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Its specificity and versatility make it an invaluable tool for in-depth studies on CCNB2, facilitating comprehensive analyses across various experimental setups and enhancing your research capabilities.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8873/91_E7_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9575/if_selected.jpg
Normal Tissue
Figure 3 Bone marrow
Hematopoietic cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8873/25829_B_6_4.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8873/25829_A_9_8.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes - Goblet cells Staining: Low Intensity: Moderate Quantity: <25% Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8873/25829_A_9_3.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000157456-CCNB2
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Specifications
- Immunogen
- Xenopus laevis cyclin B2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CCNB2
- Species Reactivity
- Human, Mouse, Non-human primate
- Clone
- X29.2
- Applications
- WB, IHC, IF, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS with 1 mg/mL BSA, 30% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IHC: 1:50-1:500, IF: 1:20-1:200, IP: 1:800
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CCNB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2373CT | Mouse Anti-CCNB2 Recombinant Antibody (clone 7C4) | IHC-P, IF, WB, FC | Mouse IgG1 |
| MRO-0412-CN | Rabbit Anti-CCNB2 Recombinant Antibody (clone CBACN-163) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-CJ187 | Rabbit Anti-CCNB2 Recombinant Antibody (VS3-CJ187) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-FY216 | Recombinant Rabbit Anti-CCNB2 Antibody (clone R05-3A6) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY335 | Anti-CCNB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1084 | Anti-Frog CCNB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1083 | Anti-Human CCNB2 Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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