Mouse Anti-NHP CDK1 Recombinant Antibody (VS-1024-XY117)
CAT#: VS-1024-XY117
This monoclonal antibody, derived from mouse, specifically targets CDK1 and is effective in human and non-human primate research. It is raised against a recombinant human CDK1 fusion protein, ensuring high specificity. With proven utility in Western blot (WB), immunofluorescence (IF), and immunoprecipitation (IP) assays, this antibody is essential for investigating CDK1's role and interactions. Its broad application range underscores its significance in detailed biological studies.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3387/79_H6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79974/1999_A5_3_selected.jpg
Normal Tissue
Figure 3 High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3387/11302_A_6_8_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3799/10153_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3799/10153_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3799/10153_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170312-CDK1
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Specifications
- Immunogen
- Recombinant human CDK1 fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- CDK1
- Species Reactivity
- Human, Non-human primate
- Applications
- WB, IF, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IF: 1:200-1:800, IP: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| MOB-0511CT | Recombinant Mouse anti-Human CDK1 Monoclonal antibody (9D6B8G20) | ELISA, FC, ICC, IF, WB | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-451CQ | Mouse Anti-CDK1 Recombinant Antibody (clone A17.1.1) | ICC, IF, IHC-P, Inhib, IP, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-452CQ | Mouse Anti-CDK1 Recombinant Antibody (clone CBL924) | IF, IHC, IP, Neut, WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0623 | Hi-Affi™ Rabbit Anti-CDK1 Recombinant Antibody (clone DS623AB) | IP, WB | Rabbit IgG |
| MOR-4517 | Hi-Affi™ Rabbit Anti-CDK1 Recombinant Antibody (clone TH25DS) | WB, IF, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY60 | AbPlus™ Anti-CDK1 Magnetic Beads (POH-1) | IP, Protein Purification | |
| VS-0724-YC1248 | AbPlus™ Anti-CDK1 Magnetic Beads (VS-0724-YC1248) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY395 | Anti-CDK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1302 | Anti-Mouse CDK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1301 | Anti-Human CDK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY1303 | Anti-Rat CDK1 Immunohistochemistry Kit | IHC |
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