Mouse Anti-CDK1 Recombinant Antibody (clone 25A3)
CAT#: MOB-2515z
This product is a mouse antibody that recognizes human CDK1. It can be used in the following applications: WB, ELISA, ICC/IF, IHC.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3387/79_H6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79974/1999_A5_3_selected.jpg
Normal Tissue
Figure 3 High expression in tonsil
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3387/11302_A_6_8_val_selected.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3799/10153_A_8_8.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3799/10153_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Spermatogonia cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3799/10153_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000170312-CDK1
❮
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Specifications
- Immunogen
- Human Cyclin-Dependent Kinase 1
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CDK1
- Species Reactivity
- Human
- Clone
- 25A3
- Applications
- WB, ELISA, ICC, IF, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Western blot, Enzyme-linked Immunosorbent Assay, Immunocytochemistry/Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- CDK1; P34CDC2; P34 Protein Kinase; CDKN1; Cell Cycle Controller CDC2; Cyclin-Dependent Kinase 1; Cell Division Cycle 2, G1 To S And G2 To M; EC 2.7.11.23; CDC28A
- UniProt ID
- P06493
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| ZG-0397F | Mouse Anti-CDK1 Recombinant Antibody (ZG-0397F) | WB | Mouse IgG |
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| CAT | Product Name | Application | Type |
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| VS-0424-XY60 | AbPlus™ Anti-CDK1 Magnetic Beads (POH-1) | IP, Protein Purification | |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-XY1303 | Anti-Rat CDK1 Immunohistochemistry Kit | IHC |
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