Mouse Anti-NHP EZH2 Recombinant Antibody (clone H.547.3) (CAT#: VS-1024-XY197)
Tailored for high-precision detection of EZH2, this mouse monoclonal antibody displays reactivity with human, mouse, non-human primate, and rat samples. The immunogen is a recombinant EZH2 protein, ensuring exceptional specificity. Optimized for Western blot (WB) applications, it offers reliable performance for analyzing EZH2 expression across a range of species, making it an indispensable tool for detailed molecular research and diagnostic investigations.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SiHa
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2008_C2_16_cr5f1a9e9d6c53d_selected.jpg
(Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2008_C2_16_cr5f1a9e9d6c53d_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/if_selected.jpg
Figure 3 IF staining of human cell line REH
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2027_D2_1_red_green.jpg
(Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2027_D2_1_red_green.jpg
Figure 4 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/582_H8_1_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/582_H8_1_red_green.jpg
Figure 5 Colon
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_9_3.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_9_3.jpg
Figure 6 Testis
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_6_6.jpg
(Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_7_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_7_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106462-EZH2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106462-EZH2
Specifications
- Immunogen
- EZH2 recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- EZH2
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- H.547.3
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC078 | PE-A*02:01/Human EZH2 (YMCSFLFNL) MHC Tetramer | FCM | |
| MHC-CN0657 | PE-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0658 | APC-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0659 | FITC-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0660 | A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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