Mouse Anti-EZH2 Recombinant Antibody (clone AC22)
CAT#: VS-0725-FY179
The Mouse Anti-EZH2 Recombinant Antibody (clone AC22) is a recombinant antibody developed against a recombinant protein corresponding to amino acids 353-451 of human EZH2. It is suitable for Chromatin Immunoprecipitation (ChIP). EZH2 is a Polycomb-group (PcG) family member that forms multimeric protein complexes essential for maintaining the transcriptional repressive state of genes. This antibody provides a valuable tool for studying epigenetic gene silencing and its role in various biological processes.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2008_C2_16_cr5f1a9e9d6c53d_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/if_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/2027_D2_1_red_green.jpg
Subcellular Location
Figure 4 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29131/582_H8_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_9_3.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9589/23664_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000106462-EZH2
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Specifications
- Immunogen
- A recombinant protein corresponding to amino acids 353-451 of human EZH2.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human EZH2
- Species Reactivity
- Human, Mouse
- Clone
- AC22
- Applications
- Chromatin Immunoprecipitation, Cleavage Under Targets & Release Using Nuclease
- Related Disease
- Cancer, Weaver syndrome, Myelodysplastic syndromes
Product Property
- Purification
- Protein A purified
- Molecular Weight
- The molecular weight of the antigen is 96 kDa.
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol
- Preservative
- 0.035% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Chromatin Immunoprecipitation: 1-3 µg per Chromatin Immunoprecipitation
Cleavage Under Targets & Release Using Nuclease: 1 µL per 50 µL reaction
Target
- Alternative Names
- WVS; ENX1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
- Gene ID
- 2146
- UniProt ID
- Q15910
- Long Name
- Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit
- Sequence Similarities
- Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylated by AKT1.
Phosphorylation by AKT1 reduces methyltransferase activity.
Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing.
- Protein Refseq
- XP_005250019.1; XP_005250020.1; XP_005250021.1; XP_011514185.1; XP_011514187.1
- Function
- Polycomb group (PcG) protein.
Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene.
Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively.
Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0> H3K27me1> H3K27me2.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "EZH2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1221 | Hi-Affi™ Rabbit Anti-EZH2 Recombinant Antibody (clone DS1221AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0905-CN | Rabbit Anti-EZH2 Recombinant Antibody (clone CBACN-338) | WB, IF, IHC | Rabbit IgG |
| VS3-QX394 | Mouse Anti-EZH2 Recombinant Antibody (clone 3D2A4) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-QX395 | Mouse Anti-EZH2 Recombinant Antibody (clone 144CT2.1.1.5) | WB, ICC, IHC | Mouse IgG1, κ |
| VS3-QX396 | Mouse Anti-EZH2 Recombinant Antibody (clone 6F-4G) | WB, IHC, FC | Mouse IgG1 |
| VS3-FY526 | Recombinant Rabbit Anti-EZH2 Antibody (clone R07-5B1) | WB, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0657 | PE-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0658 | APC-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0659 | FITC-A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-CN0660 | A*02:01/Human EZH2 (SQADALKYV) MHC Tetramer | FCM, IHC | |
| MHC-YF275 | A*02:01/Human EZH2 (YMCSFLFNL) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY197 | Mouse Anti-NHP EZH2 Recombinant Antibody (clone H.547.3) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2396 | Anti-EZH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2397 | Anti-Mouse EZH2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY2398 | Anti-Rat EZH2 Immunohistochemistry Kit | IHC |
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