Mouse Anti-NHP IRS1 Recombinant Antibody (VS-1024-XY286)
CAT#: VS-1024-XY286
This mouse monoclonal antibody is meticulously developed for the specific detection of IRS1, with reactivity spanning human, mouse, non-human primate, and rat species. The immunogen, a synthetic peptide derived from the IRS1 protein, ensures targeted accuracy. Optimized for use in Western blot (WB), this antibody provides reliable performance for investigating IRS1 expression and its biological role in multiple research settings.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46433/824_C8_5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/963_D12_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/19658_B_9_5.jpg
Normal Tissue
Figure 4 Cerebellum
Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/19658_B_9_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/19658_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/19658_A_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169047-IRS1
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Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- IRS1
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800
Target
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Related Signaling Pathways
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
PPAR Signaling Pathway
Related Diseases
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IRS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2476z | Mouse Anti-IRS1 Recombinant Antibody (clone 36A2) | WB, FC, IHC | Mouse IgG2a |
| MRO-0874-CN | Recombinant Rabbit Anti-IRS1 Monoclonal Antibody (CBACN-326) | WB, IF, IHC | Rabbit IgG |
| FAMAB-1375CQ | Mouse Anti-IRS1 Recombinant Antibody (clone IRS 8-63) | IB, IP, WB | Mouse IgG1 |
| VS3-CJ905 | Rabbit Anti-IRS1 Recombinant Antibody (VS3-CJ905) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1540CQ | Recombinant Mouse Anti-IRS1 Antibody (CBL216) | Inhib | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1861 | Hi-Affi™ Recombinant Rabbit Anti-IRS1 Monoclonal Antibody (DS1861AB) | WB | IgG |
| MOR-4635 | Hi-Affi™ Recombinant Rabbit Anti-IRS1 Monoclonal Antibody (TH148DS) | WB, IHC-P | IgG |
| MOR-4689 | Hi-Affi™ Recombinant Rabbit Anti-IRS1 Monoclonal Antibody (TH203DS) | WB, IF, ICC, FC | IgG |
| MOR-0045-FY | Rabbit Anti-IRS1 Recombinant Antibody (clone AFY0016) | IHC, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4426 | PE-A*02:01/Human IRS1 (GLENGLNYI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3599 | Anti-IRS1 Immunohistochemistry Kit | IHC |
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