Rabbit Anti-IRS1 Recombinant Antibody (VS3-CJ905)
CAT#: VS3-CJ905
This product is a rabbit antibody that recognizes human IRS1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46433/824_C8_5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/963_D12_1_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/19658_B_9_5.jpg
Normal Tissue
Figure 4 Cerebellum
Cells in granular layer Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in molecular layer Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Purkinje cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/19658_B_9_8.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/19658_A_7_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5261/19658_A_7_5.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000169047-IRS1
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IRS1
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry.
Target
- Alternative Names
- HIRS-1
- Gene ID
- 3667
- UniProt ID
- P35568
- Cellular Localization
- Caveola, Cytoplasm, Cytosol, Nucleoplasm, Nucleus, Plasma membrane
- Post Translation Modifications
- Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity).
Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).
Phosphorylated by ALK. Phosphorylated at Ser-270, Ser-307, Ser-636 and Ser-1101 by RPS6KB1; phosphorylation induces accelerated degradation of IRS1 (PubMed:18952604).
Phosphorylated on tyrosine residues in response to insulin (PubMed:23401856).
In skeletal muscles, dephosphorylated on Tyr-612 by TNS2 under anabolic conditions; dephosphorylation results in the proteasomal degradation of IRS1 (PubMed:23401856).
Ubiquitinated by the Cul7-RING(FBXW8) complex in a mTOR-dependent manner, leading to its degradation: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2).
- Protein Refseq
- NP_005535.1
- Function
- May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
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Related Signaling Pathways
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
PPAR Signaling Pathway
Related Diseases
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "IRS1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2476z | Mouse Anti-IRS1 Recombinant Antibody (clone 36A2) | WB, FC, IHC | Mouse IgG2a |
| MRO-0874-CN | Recombinant Rabbit Anti-IRS1 Monoclonal Antibody (CBACN-326) | WB, IF, IHC | Rabbit IgG |
| FAMAB-1375CQ | Mouse Anti-IRS1 Recombinant Antibody (clone IRS 8-63) | IB, IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1540CQ | Recombinant Mouse Anti-IRS1 Antibody (CBL216) | Inhib | IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1861 | Hi-Affi™ Recombinant Rabbit Anti-IRS1 Monoclonal Antibody (DS1861AB) | WB | IgG |
| MOR-4635 | Hi-Affi™ Recombinant Rabbit Anti-IRS1 Monoclonal Antibody (TH148DS) | WB, IHC-P | IgG |
| MOR-4689 | Hi-Affi™ Recombinant Rabbit Anti-IRS1 Monoclonal Antibody (TH203DS) | WB, IF, ICC, FC | IgG |
| MOR-0045-FY | Rabbit Anti-IRS1 Recombinant Antibody (clone AFY0016) | IHC, WB, Inhib | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4426 | PE-A*02:01/Human IRS1 (GLENGLNYI) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY286 | Mouse Anti-NHP IRS1 Recombinant Antibody (VS-1024-XY286) | WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3599 | Anti-IRS1 Immunohistochemistry Kit | IHC |
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