Mouse Anti-NHP MIEN1 Recombinant Antibody (clone OTI2D2) (CAT#: VS-1024-XY549)
Engineered for the specific detection of MIEN1, this monoclonal antibody exhibits broad reactivity with dog, human, mouse, non-human primate, and rat samples. The antibody was generated using a full-length human recombinant protein as the immunogen, ensuring high specificity. It is optimally designed for applications including Western blot, immunohistochemistry, and flow cytometry. With its robust performance, this antibody serves as a vital tool for elucidating the roles of MIEN1 in various biological contexts, contributing significantly to advancements in understanding cellular dynamics and disease mechanisms.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line OE19
(Immunofluorescent staining of human cell line OE19 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56650/1971_G6_5_selected.jpg
(Immunofluorescent staining of human cell line OE19 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/56650/1971_G6_5_selected.jpg
Figure 2 IHC staining of human testis
(Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/ihc_selected.jpg
(Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/ihc_selected.jpg
Figure 3 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/140064_A_8_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/140064_A_8_3.jpg
Figure 4 Liver
(Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/140064_A_8_4.jpg
(Hepatocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/140064_A_8_4.jpg
Figure 5 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/140064_A_8_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/140064_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/140064_A_6_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/61344/140064_A_6_6.jpg
Figure 7 RNA cell line category: Group enriched (OE19, SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141741-MIEN1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000141741-MIEN1
Specifications
- Immunogen
- Full length human recombinant protein of MIEN1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MIEN1
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- OTI2D2
- Applications
- WB, IHC, FC
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, FC: 1:100
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1340CL | Anti-Human MIEN1 Recombinant Antibody (1B2) | ELISA |
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For Research Use Only. Not For Clinical Use.
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