Mouse Anti-NHP NUB1 Recombinant Antibody (clone OTI4H2) (CAT#: VS-1024-XY355)
This mouse-derived antibody against NUB1 is engineered to recognize a human recombinant protein fragment, ensuring exceptional specificity. It demonstrates extensive reactivity with dog, human, non-human primate, and rat samples. Ideal for a range of applications, including Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and Flow Cytometry (FC), this antibody is a versatile asset for exploring the roles of NUB1 in cellular processes. With its robust performance, it facilitates in-depth investigations into cellular signaling and regulatory mechanisms, providing a crucial resource for advancing research in molecular biology.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50722/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/50722/if_selected.jpg
Figure 2 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_9_3.jpg
Figure 3 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_9_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_9_4.jpg
Figure 4 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_9_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_9_5.jpg
Figure 5 Testis
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_6_6.jpg
(Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_6_6.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000013374-NUB1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000013374-NUB1
Specifications
- Immunogen
- Human recombinant protein fragment
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- NUB1
- Species Reactivity
- Dog, Human, Non-human primate, Rat
- Clone
- OTI4H2
- Applications
- WB, IHC, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, IF: 1:100, FC: 1:100
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2755z | Mouse Anti-NUB1 Recombinant Antibody (clone 23G2) | WB, FC, ICC, IF, IHC | Mouse IgG2a |
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