Mouse Anti-NHP NUB1 Recombinant Antibody (clone OTI4H2)
CAT#: VS-1024-XY355
This mouse-derived antibody against NUB1 is engineered to recognize a human recombinant protein fragment, ensuring exceptional specificity. It demonstrates extensive reactivity with dog, human, non-human primate, and rat samples. Ideal for a range of applications, including Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and Flow Cytometry (FC), this antibody is a versatile asset for exploring the roles of NUB1 in cellular processes. With its robust performance, it facilitates in-depth investigations into cellular signaling and regulatory mechanisms, providing a crucial resource for advancing research in molecular biology.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/50722/if_selected.jpg
Normal Tissue
Figure 2 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_9_3.jpg
Normal Tissue
Figure 3 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_9_4.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_9_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21220/129657_A_6_6.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000013374-NUB1
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Specifications
- Immunogen
- Human recombinant protein fragment
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- NUB1
- Species Reactivity
- Dog, Human, Non-human primate, Rat
- Clone
- OTI4H2
- Applications
- WB, IHC, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, IF: 1:100, FC: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NUB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2755z | Mouse Anti-NUB1 Recombinant Antibody (clone 23G2) | WB, FC, ICC, IF, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2510 | Hi-Affi™ Recombinant Rabbit Anti-NUB1 Monoclonal Antibody (DS2510AB) | WB, ICC, IF, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5026 | Anti-NUB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5027 | Anti-Mouse NUB1 Immunohistochemistry Kit | IHC |
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