Rabbit Anti-NHP NUMB Recombinant Antibody (clone T.873.4)
CAT#: VS-1024-XY350
This innovative antibody, aimed at NUMB, is engineered for exceptional reactivity with human, mouse, non-human primate, and rat samples. The immunogen, a synthetic peptide of NUMB, enhances specificity and binding affinity, making it ideal for a range of applications including Western Blotting (WB), Immunofluorescence (IF), and Immunoprecipitation (IP). Its advanced design not only facilitates detailed investigation into cellular signaling and differentiation but also empowers researchers to unravel the intricacies of developmental biology. Employ this rabbit-derived antibody to elevate your research to new heights.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2874/1766_B2_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11478/if_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11478/32627_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11478/32627_A_7_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11478/32627_A_9_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11478/32627_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11478/32627_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000133961-NUMB
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Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- NUMB
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- T.873.4
- Applications
- WB, IF, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800, IF: 1:400, IP: 1:100
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "NUMB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2520 | Hi-Affi™ Recombinant Rabbit Anti-NUMB Monoclonal Antibody (DS2520AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1119-CN | Recombinant Rabbit Anti-NUMB Monoclonal Antibody (JM10-023) | WB, IF, IHC | Rabbit IgG |
| VS3-WK527 | Rabbit Anti-NUMB Recombinant Antibody (clone JM10-023) | WB, ICC, IHC | Rabbit IgG |
| VS3-FY1077 | Recombinant Rabbit Anti-NUMB Antibody (clone R09-2I5) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC851 | Rabbit Anti-NUMB Recombinant Antibody (VS13-YC851) | WB, ICC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC153 | AbPlus™ Anti-NUMB Magnetic Beads (VS-0724-YC153) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5046 | Anti-NUMB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5047 | Anti-Mouse NUMB Immunohistochemistry Kit | IHC |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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