Rabbit Anti-NHP PKM Recombinant Antibody (VS-1024-XY393) (CAT#: VS-1024-XY393)
This meticulously crafted rabbit monoclonal antibody is specifically designed to target PKM, demonstrating robust reactivity with human, mouse, non-human primate, and rat samples. Optimized for applications such as Western blotting and immunofluorescence, it serves as a reliable tool for the detection and visualization of PKM in various experimental contexts. Researchers can harness this antibody to explore the critical roles of PKM in metabolic regulation and cellular energy pathways, thereby advancing our understanding of its contributions to physiological processes and its potential implications in diverse disease conditions.
Specific Inquiry
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/283_B10_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/283_B10_1_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/if_selected.jpg
Figure 3 IHC staining of human fallopian tube
(Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/ihc_selected.jpg
(Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/ihc_selected.jpg
Figure 4 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/ihc_selected.jpg
Figure 5 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_B_8_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_B_8_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_3.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_3.jpg
Figure 7 Liver
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_4.jpg
(Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_4.jpg
Figure 8 Kidney
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_5.jpg
(Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_5.jpg
Figure 9 Testis
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_4_6.jpg
(Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_4_6.jpg
Figure 10 Lymph node
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_8_8.jpg
(Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_8_8.jpg
Figure 11 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067225-PKM
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067225-PKM
Specifications
- Immunogen
- Synthetic peptide
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- PKM
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Applications
- WB, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M HEPES, pH 7.5, with 0.15 M NaCl, 100 µg/mL BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800-1:5,000, IF: 1:50-1:100
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2125-CN | Rabbit Anti-PKM Polyclonal Antibody (MRO-2125-CN) | WB, IF, IHC | Rabbit IgG |
| ZG-0378C | Mouse Anti-PKM Recombinant Antibody (ZG-0378C) | WB, ICC | Mouse IgG |
| ZG-0379C | Mouse Anti-PKM Recombinant Antibody (ZG-0379C) | WB, ICC | Mouse IgG |
| VS3-CJ43 | Mouse Anti-PKM Recombinant Antibody (VS3-CJ43) | WB, ELISA | Mouse IgG |
| VS3-XY3466 | Rabbit Anti-PKM Recombinant Antibody (clone R09-7E7) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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