Mouse Anti-NHP PRMT5 Recombinant Antibody (clone PRMT5-21)
CAT#: VS-1024-XY409
This monoclonal antibody is expertly crafted to target PRMT5, showcasing broad reactivity with bovine, hamster, human, mouse, non-human primate, and rat samples. Derived from human PRMT5 as its immunogen, it guarantees specificity and reliable performance. Designed primarily for Western blotting applications, this antibody serves as an essential tool for researchers probing the multifaceted roles of PRMT5 in epigenetic regulation and cellular signaling. Its exceptional versatility and precision make it indispensable for advancing our understanding of PRMT5's impact across various biological systems.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64708/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_8_2_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/5_F2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/4_F2_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_7_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100462-PRMT5
❮
❯
Specifications
- Immunogen
- Human PRMT5
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- PRMT5
- Species Reactivity
- Bovine, Hamster, Human, Mouse, Non-human primate, Rat
- Clone
- PRMT5-21
- Applications
- WB
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 0.01 M PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 2 µg/mL
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-1024-XY409. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PRMT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-481 | Recombinant Human Anti-Human PRMT5 Antibody Fab Fragment, clone D245 | IF, ELISA, IP-MS, ChIP | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2869 | Hi-Affi™ Recombinant Rabbit Anti-PRMT5 Monoclonal Antibody (DS2869AB) | WB, IP, IHC-P, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1268-CN | Recombinant Mouse Anti-PRMT5 Monoclonal Antibody (7-C2) | WB, IHC | Mouse IgG2a |
| MRO-1269-CN | Recombinant Rabbit Anti-PRMT5 Monoclonal Antibody (CBACN-458) | WB, IF, IHC | Rabbit IgG |
| MRO-2138-CN | Rabbit Anti-PRMT5 Polyclonal Antibody (MRO-2138-CN) | WB, IF | Rabbit IgG |
| ZG-0525U | Rabbit Anti-PRMT5 Recombinant Antibody (clone 2H4) | ELISA, WB, IHC | Rabbit IgG |
| VS3-CJ126 | Rabbit Anti-PRMT5 Recombinant Antibody (VS3-CJ126) | WB, ICC, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1762 | Anti-PRMT5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5724 | Anti-Mouse PRMT5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5723 | Anti-Human PRMT5 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: ELISA, Neut, IF, IP, FC, FuncS
-2-1.png)
Application: IP, IF, FuncS, FC, Neut, ELISA, IHC
Application: Neut, ELISA, FuncS
Application: ELISA, IHC, FC, IP, IF, FuncS
-3.jpg)
Application: IB, FC, ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
