Rabbit Anti-PRMT5 Recombinant Antibody (clone 2H4) (CAT#: ZG-0525U)
Rabbit Anti-PRMT5 Antibody (clone 2H4) displayed high specificity against the PRMT5 protein of Human.
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WB
Figure 1 Rabbit Anti-PRMT5 Antibody (ZG-0525U) in WB
Western Blot
Positive WB detected in: Raji whole cell lysate, Hela whole cell lysate, HL60 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate
All lanes: PRMT5 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73, 72, 54, 68, 67 kDa
Observed band size: 73 kDa
IHC
Figure 2 Rabbit Anti-PRMT5 Antibody (ZG-0525U) in IHC
IHC image of ZG-0525U diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human PRMT5
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRMT5
- Species Reactivity
- Human
- Clone
- 2H4
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Mantle Cell Lymphoma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Protein arginine N-methyltransferase 5; EC 2.1.1.320; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB1 homolog; SKB1Hs [Cleaved into: Protein arginine N-methyltransferase 5; N-terminally processed]; PRMT5; HRMT1L5 IBP72 JBP1 SKB1
- Gene ID
- 10419
- UniProt ID
- O14744
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-481 | Recombinant Human Anti-Human PRMT5 Antibody Fab Fragment, clone D245 | IF, ELISA, IP-MS, ChIP | Fab |
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For Research Use Only. Not For Clinical Use.
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