Rabbit Anti-PRMT5 Recombinant Antibody (clone 2H4) (CAT#: ZG-0525U)
Rabbit Anti-PRMT5 Antibody (clone 2H4) displayed high specificity against the PRMT5 protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-PRMT5 Antibody (ZG-0525U) in WB
Western Blot
Positive WB detected in: Raji whole cell lysate, Hela whole cell lysate, HL60 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate
All lanes: PRMT5 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 73, 72, 54, 68, 67 kDa
Observed band size: 73 kDa
IHC
Figure 2 Rabbit Anti-PRMT5 Antibody (ZG-0525U) in IHC
IHC image of ZG-0525U diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human PRMT5
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PRMT5
- Species Reactivity
- Human
- Clone
- 2H4
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Mantle Cell Lymphoma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Protein arginine N-methyltransferase 5; EC 2.1.1.320; 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; Jak-binding protein 1; Shk1 kinase-binding protein 1 homolog; SKB1 homolog; SKB1Hs [Cleaved into: Protein arginine N-methyltransferase 5; N-terminally processed]; PRMT5; HRMT1L5 IBP72 JBP1 SKB1
- Gene ID
- 10419
- UniProt ID
- O14744
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
Rabbit Monoclonal Antibody Products
IgG Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-481 | Recombinant Human Anti-Human PRMT5 Antibody Fab Fragment, clone D245 | IF, ELISA, IP-MS, ChIP | Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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