Recombinant Human Anti-Human PRMT5 Antibody Fab Fragment, clone D245
CAT#: DrMAB-481
Recombinant Human Antibody binds selectively to Human PRMT5, expressed in E. coli.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64708/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_8_2_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/5_F2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/4_F2_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_7_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100462-PRMT5
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Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Type
- Fab
- Antibody Isotype
- IgG1 Fab
- Specificity
- reacts with human PRMT5
- Species Reactivity
- Human
- Clone
- D245
- Applications
- IF; ELISA; IP-MS; ChIP
Product Property
- Storage
- short term – store at 4°C (over 6 months), long term - PBS -20°C or -80°C
Target
- Alternative Names
- PRMT5; protein arginine methyltransferase 5; JBP1; SKB1; IBP72; SKB1Hs; HRMT1L5; protein arginine N-methyltransferase 5; 72 kDa ICln-binding protein; HMT1 hnRNP methyltransferase-like 5; SKB1 homolog; histone-arginine N-methyltransferase PRMT5; jak-bindin
- Gene ID
- 10419
- UniProt ID
- O14744
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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