Anti-PRMT5 Immunohistochemistry Kit
CAT#: VS-0325-XY1762
Our PRMT5 IHC Kit comes with a full suite of reagents, ensuring a seamless immunohistochemistry experiment. Engineered for ease of use, it has clear instructions for trouble - free handling. It is compatible with both paraffin - cut and frozen tissue slices, enabling reliable PRMT5 detection. This is valuable for research on PRMT5 - mediated symmetric dimethylation and its impact on biological pathways.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/64708/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_8_2_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/5_F2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5525/4_F2_1_blue_red_green.jpg
Normal Tissue
Figure 6 Cerebral cortex
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuronal cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_B_7_5.jpg
Normal Tissue
Figure 7 Colon
Glandular cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_7_3.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12459/27939_A_9_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100462-PRMT5
❮
❯
Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human
- Target
- PRMT5
- Primary Antibody
- Rabbit Anti-PRMT5 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PRMT5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-481 | Recombinant Human Anti-Human PRMT5 Antibody Fab Fragment, clone D245 | IF, ELISA, IP-MS, ChIP | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2869 | Hi-Affi™ Recombinant Rabbit Anti-PRMT5 Monoclonal Antibody (DS2869AB) | WB, IP, IHC-P, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1268-CN | Recombinant Mouse Anti-PRMT5 Monoclonal Antibody (7-C2) | WB, IHC | Mouse IgG2a |
| MRO-1269-CN | Recombinant Rabbit Anti-PRMT5 Monoclonal Antibody (CBACN-458) | WB, IF, IHC | Rabbit IgG |
| MRO-2138-CN | Rabbit Anti-PRMT5 Polyclonal Antibody (MRO-2138-CN) | WB, IF | Rabbit IgG |
| ZG-0525U | Rabbit Anti-PRMT5 Recombinant Antibody (clone 2H4) | ELISA, WB, IHC | Rabbit IgG |
| VS11-QX124 | Human Anti-PRMT5 Recombinant Antibody (clone RAB-C136) | ELISA, IF, IP-MS, ChIP | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY409 | Mouse Anti-NHP PRMT5 Recombinant Antibody (clone PRMT5-21) | WB | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5724 | Anti-Mouse PRMT5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5723 | Anti-Human PRMT5 Immunohistochemistry Kit | IHC |
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